中国畜牧兽医2016，Vol.43Issue(10)：2541-2546,6.DOI:10.16431/j.cnki.1671-7236.2016.10.005
猪传染性胃肠炎病毒截短S基因原核表达及免疫原性初步检测
Prokaryotic Expression of Truncated S Gene of Porcine Transmissible Gastroenteritis Virus and Preliminary Test of Immunogenicity
摘要
Abstract
To monitor TGEV antibody in pig,major antigenic fragments of TGE B/C zone was amplified by PCR,purified PCR product and pET-32a which were separately digested,and were connected and transformed into the BL21 competent cells.We picked out successfully transformed bacterial colonies to amplify by PCR,and put the remained bacterial colonies into LB solution to culture overnight,which was extracted to get recombinant plasmid and sequenced.We cultivated right sequenced bacteria to induce protein to express by IPTG,there was a right target band in 37 ku,which was purified by cutting polyacrylamide gel,then detected by Western blotting with homemade anti-rabbit serum,specific band (about 37 ku)could finally be seen in the right posi-tion,which proved that this recombinant protein was immunogenic.It would profitably create ELISA-kit to supervise TGEV antibody,and effectively provide some relevant value in immune situation of pig.关键词
猪传染性胃肠炎病毒/B/C区/截短表达Key words
TGEV/B/C zone/truncated expression分类
生物科学引用本文复制引用
郑仲华,魏楚丹,赵爽,李盼畔,陈金顶,杨佩,赵明,丘峰,吴祖庆,张仕云,柳俏凡,何洪洲,洪纯丹..猪传染性胃肠炎病毒截短S基因原核表达及免疫原性初步检测[J].中国畜牧兽医,2016,43(10):2541-2546,6.基金项目
广东省省级科技计划项目(2015B020230009) （2015B020230009）
