中国畜牧兽医2016，Vol.43Issue(10)：2560-2565,6.DOI:10.16431/j.cnki.1671-7236.2016.10.008
犬α-干扰素蛋白在不同表达载体中的生物学活性比较
The Comparison of Canine α-Interferon Antiviral Activity in Different Expression Vectors
摘要
Abstract
According to the sequence of canine interferon-α (CaIFN-α)gene published in Gen-Bank,primers were designed,synthesized and introduced EcoRⅠand HindⅢ restriction sites.U-sing canine liver DNA as a template,CaIFN-α gene was amplified by PCR,then the gene was cloned into pBV220 and pET-32a(+)expression vector,and transformed into the BL21(DE3)ex-pression system.Recombinant fusion protein was analysed by SDS-PAGE and Western blotting. Protein was purified and the antiviral activity was detected by MDCK/VSV cytopathic inhibition assay.The results showed that pBV220-α-IFN recombinant protein has a low activity,but the ac-tivity of pET32a-α-IFN recombinant protein was up to 2.56×10 6 U/mL.This study laid a founda-tion for production of highly active IFN.关键词
犬/α-干扰素/原核表达/生物学活性Key words
canine/interferon-α/prokaryotic expression/antiviral activity分类
生物科学引用本文复制引用
刘欢,金红岩,侯强,史秋梅,董瑞凯,张通明,侯绍华,沈萍..犬α-干扰素蛋白在不同表达载体中的生物学活性比较[J].中国畜牧兽医,2016,43(10):2560-2565,6.基金项目
公益性行业(农业)项目“宠物疫病快速诊断与疫苗研究与示范”(201303042) （农业）
