中国畜牧兽医2016，Vol.43Issue(10)：2634-2639,6.DOI:10.16431/j.cnki.1671-7236.2016.10.018
4种抗原定量方法观察牛支原体培养特征
Observation of the Growth Trends of Mycoplasma bovis with Four Different Antigen Quantitation Methods
摘要
Abstract
To explore the antigen harvest time of Mycoplasma bovis (M.bovis )and the antigen quantitation alternative method,M.bovis 08M strain was inoculated in the Thiaucourt’s medium. Four growth curve plottings were made by measuring color change units (CCU),colony forming units (CFU),protein concentration and nucleic acid levels.Both the results of CCU and CFU counts showed that the growth of M.bovis was divided into four phases,the logarithmic phase ap-peared after being cultrured 10 h,the stationary phase appeared at 30 h with the highest number of viable cells up to 1.0×10 8 CCU/mL and 7.7×10 7 CFU/mL,and the decline phase started at 75 h.The protein concentration of M.bovis increased rapidly from 15 to 35 h,reached 72.06 μg/mL at 35 h,then maintained at 58.38 to 70.65 μg/mL.The nucleic acid levels of M.bovis increased rapidly from 15 h,and the cycle threshold (Ct)values were maintained between 15.32 to 17.84 after 25 h.These results indicated that there was a good correlation between the protein concen-tration and viable count of M.bovis at the early stationary phase,which was the best time period to harvest antigen.The protein concentration determination could be an alternative method to quantify antigen contents of M.bovis when preparing inactivated M.bovis vaccine.关键词
牛支原体/抗原定量/颜色变化单位/菌落形成单位/蛋白浓度/核酸含量Key words
Mcoplasma bovis/antigen quantitative/color change units/colony forming units/pro-tein concentration/nucleic acid contents分类
农业科技引用本文复制引用
王展慧,赵萍,陈胜利,郝华芳,秦明明,王绍伟,刘永生,储岳峰..4种抗原定量方法观察牛支原体培养特征[J].中国畜牧兽医,2016,43(10):2634-2639,6.基金项目
国家科技支撑计划(2015BAD12B02)；甘肃省科技支撑计划(1204NKCA071)；兰州市城关区科技计划(2012-2-1) （2015BAD12B02）
