中国畜牧兽医2016，Vol.43Issue(10)：2666-2671,6.DOI:10.16431/j.cnki.1671-7236.2016.10.023
猪ICSI胚胎制备条件的优化
The Optimization of Preparating Conditions on Pig ICSI Embryos
摘要
Abstract
In order to optimize the preparating conditions on pig intracytoplasmic sperm injection (ICSI)embryos,the Piezo operating system was used in this experiment and the embryonic sur-vival rate,cleavage rate and cell numbers of blastocysts developed in various stages were measured as traditional and Piezo operating system,the concentration of CB,the operating temperature with Piezo operating system and different sperm processing method in preparating pig ICSI embryos. The results showed that Piezo operating system could significantly improve the survival rate and cleavage rate of injected oocytes (P <0.05),but there was no significant effect on blastocyst rate. There was a slight damage to oocyte added CB in the operating fluid,and adding 7.5 μg/mL CB was most concentration,30 ℃ was most appropriate operating temperature,but there was no sig-nificant effect of early development on pig ICSI embryos with breaking sperm tail with ultrasonic wave.The results suggested that Piezo operating system could significantly improve the survival rate.The results suggested that breaking sperm tail with ultrasonic wave,adding 7.5 μg/mL CB in operating fluid and 30 ℃ of operating temperature and using Piezo operating system was more suitable for preparating pig ICSI embryos.关键词
猪/胞浆内单精子注射/制备条件Key words
pig/ICSI/preparating conditions分类
农业科技引用本文复制引用
张立苹,刘西梅,李莉,肖红卫,华文君,毕延震,郑新民..猪ICSI胚胎制备条件的优化[J].中国畜牧兽医,2016,43(10):2666-2671,6.基金项目
转基因生物新品种培育重大专项(2016ZX08006001-005、2016ZX08006002-006、2016ZX08010003-006)；湖北省农业科技创新中心(2016-620-000-001-027)；湖北省科技支撑计划(2014BBB010)；湖北省农科院青年基金(2014NKYJJ02、2016NKYJJ19) （2016ZX08006001-005、2016ZX08006002-006、2016ZX08010003-006）
