中国畜牧兽医2016，Vol.43Issue(10)：2680-2687,8.DOI:10.16431/j.cnki.1671-7236.2016.10.025
中甸牦牛mtDNACytb基因遗传多样性及系统进化分析
Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak
摘要
Abstract
In order to investigate the genetic diversity and the origin of evolutionary relationship of Zhongdian yak,we analyzed the complete sequence of 15 individuals Cytb gene,its sequence poly-morphism was analyzed,and the phylogenetic tree was constructed.The results showed that the length of the nucleotide sequence were 1 140 bp,with nucleotide frequencies of 26.3%,31.8%, 13.1% and 28.8% for T,A,G and C,respectively.Three haplotypes were identified of 15 individ-uals,with 3 polymorphic sites,including two conversions,one transversion,haplotype diversity was 0.2571 and nucleotide diversity was 0.00035.Phylogenetic analysis suggested that Zhongdian yak and Bos mutusc clustered firstly,then gathered with Bison bison ,which indicated that there were high genetic similarity and closer genetic relationship,genetic similarity with other cattle ge-nus was relatively low.Combining with the proof of molecular biology and paleontology,the result supported the point that Bos grunniens and Bos mutus were classified as an alone genus in Bovi-nae.关键词
中甸牦牛/细胞色素b基因/系统进化/遗传多样性Key words
Zhongdian yak/Cytb gene/phylogenetic evolution/genetic diversity分类
农业科技引用本文复制引用
涂世英,宋娜娜,柴志欣,钟金城..中甸牦牛mtDNACytb基因遗传多样性及系统进化分析[J].中国畜牧兽医,2016,43(10):2680-2687,8.基金项目
四川省科技计划项目(2015JY0248) （2015JY0248）
