中国畜牧兽医2016，Vol.43Issue(10)：2716-2723,8.DOI:10.16431/j.cnki.1671-7236.2016.10.030
不同类型CpG基序对细粒棘球蚴Eg95抗原的免疫增强作用研究
Immune Enhancement Activity of Different Types of CpG Motifs on the Recombinant Eg95 Antigen of Echinococus granulosus
摘要
Abstract
To study the immune enhancement activity of CpG motifs on the recombinant Eg95 an-tigen of Echinococcus granulosus ,Escherichia coli BL21 (DE3 )containing pET-32a-3Eg95 was grown in LB medium,and IPTG was then added for induction.The recombinant Eg95 protein was expressed and later harvested by affinity chromatography.The obtained antigen was mixed either with the conventional adjuvant Quli-A or with different types of CpG motifs (pUC18-CpG or CpG ODN),to prepare vaccine candidates for mice immunization.The average levels of serum IgG anti-body were tested by indirect ELISA and the quantification of cytokine expression were determined by Real-time quantitative PCR after in vitro stimulation.The results identified that the size of the recombinant protein was 56 ku as predicted,and the recombinant protein could be recognized by serum from Echinococcus granulosus-infected sheep.The average levels of serum IgG antibody (D 450 nm )of the pUC18-CpG and CpG ODN groups on 14,28 and 42 d were 3.10,3.03,3.22 and 2.98,3.12,3.27,respectively.Antibody levels from both CpG groups were significantly higher compared to the conventional adjuvant Quli-A group (P <0.05)The average level of serum IgG antibody in CpG ODN group was a little higher than that in pUC18-CpG group(P >0.05).After stimulation by recombinant protein 3Eg95,the relative expression levels of IFN-β,IFN-γ and TNF-αof pUC18-CpG and CpG ODN groups were 6.88,2.35,6.28 and 5.03,2.85,7.07,respec-tively,and both were higher than that of the Quli-A group (P <0.05).The results indicated that pUC18-CpG and CpG ODN had significant immune enhancement property on Eg95 antigen in both humoral and cellular immune response,and the immune enhancement activity of CpG ODN was slightly better than that of the pUC18-CpG.Hence,pUC18-CpG and CpG ODN could be used as efficient immune adjuvants enhancer in future vaccines against Echinococcus granulosus infec-tion.关键词
细粒棘球蚴/pUC18-CpG/CpG ODN/免疫刺激活性Key words
Echinococus granulosus/pUC18-CpG/CpG ODN/immune enhancer分类
农业科技引用本文复制引用
董瑞凯,郭晓宇,袁维峰,贾红,鑫婷,侯绍华,朱鸿飞..不同类型CpG基序对细粒棘球蚴Eg95抗原的免疫增强作用研究[J].中国畜牧兽医,2016,43(10):2716-2723,8.基金项目
国家自然科学基金(31402232) （31402232）
