中国畜牧兽医2016，Vol.43Issue(10)：2724-2729,6.DOI:10.16431/j.cnki.1671-7236.2016.10.031
抗鸡球虫药常山口服液对小鼠的急性毒性作用评价
Study on Acute Toxicity Test of Anticoccidial Oral Liquid from Dichroa febrifuga in Mice
摘要
Abstract
The purpose of this study was to evaluate drug safety of Dichroa febrifuga oral liquid (DFOL),and the acute toxicity test of DFOL was established in mice based on modified Karber’s method and dose increasing method,and histopathological examination had been applied to ex-plore the main target organs of its toxic injury.The dosage was determined by preliminary experi-ment,a total of 60 Kunming mice were selected and divided randomly into one control group and five experimental groups,DFOL was administered by intragastric gavage at five different doses (1.00,1.40,1.96,2.74,3.84 g/(kg·BW)),and observed for 7 days continuously,the LD50 value and 95% confidence interval of LD50 were determined by the modified Karbers method.It showed that LD50 was 2.0961 g/(kg · BW ) and its 95% confidence interval was 1.7414 to 2.5429 g/(kg·BW).The liver and kidney of some mice in 1.96,2.74 and 3.84 g/(kg · BW) drug groups appeared severe edema,congestion and white necrotic foci by necropsy.Acute liver injury was found in mice by histopathological examination and the liver cell showed degenerative change,kidney damage showed renal congestion,interstitial edema,renal tubule epithelial cloudy swelling and degeneration.The liver was damaged seriously in the main organ and the main toxic organ of DFOL was liver.The test showed that the toxicity of DFOL was lower and it had high security in a conventional doses for clinical anti-coccidiosis application,however,high dose,long course of medication had toxicity,and the toxicity of DFOL should be analyzed and evaluated com-bining with long-term toxicity tests and many other inspection results.关键词
常山口服液/急性毒性/组织病理/靶器官Key words
Dichroa febrifuga oral liquid (DFOL)/acute toxicity/tissue pathology/target organ分类
农业科技引用本文复制引用
王玲,郭志廷,张晓松,林春全,罗小琴,杨峰,杨珍..抗鸡球虫药常山口服液对小鼠的急性毒性作用评价[J].中国畜牧兽医,2016,43(10):2724-2729,6.基金项目
中央级公益性科研院所专项资金项目(1610322011004)；横向委托项目(2015-007) （1610322011004）
