中国畜牧兽医2016，Vol.43Issue(10)：2756-2761,6.DOI:10.16431/j.cnki.1671-7236.2016.10.036
贵州矮马鼠伤寒沙门氏菌的分离鉴定及其致病力研究
Isolation,Identification and Pathogenicity of Salmonella typhimurium from Guizhou Pony
摘要
Abstract
To investigate the reason of the diarrhea in Guizhou pony,we used the feces of pony as experimental material to isolate and detect pathogenic bacteria.S6 strain was isolated from SS and XLD medium,and identified using Gram staining,biochemical tests and molecular phylogeny methods.The results showed that S6 strain could growth on SS and XLD medium,and the Gram staining was negative.Biochemical test suggested that its phenotype features were accordance with Salmonella .The 1 6S rRNA gene sequence of S6 strain was determined in a nucleotide se-quence identity of 99% with Salmonella typhimurium ATCC 1331 1 (NR_1 1 9108).Based on the morphological and molecular phylogenetic results,the strain was identified as Salmonella typhi-murium S6 strain.It was virulent to mice with the median lethal dose (LD50 )of 4.71×10 2 CFU. Then,we amplified the invasion protein A (invA)gene by PCR method.The invA gene isolated from S6 strain contained 6 to 8 bp different from the known gene,which resulted in only one ami-no acid substitution.The mutant sites of invA gene might attribute to the pathogenicity of S6 strain.The detection rate of Salmonella was 57% in Guizhou pony population.It was inferred that the diarrhea in Guizhou pony might be caused by virulent Salmonella typhimurium .关键词
贵州矮马/鼠伤寒沙门氏菌/侵袭蛋白A基因/致病力Key words
Guizhou pony/Salmonella/invasive protein A gene/pathogenicity分类
农业科技引用本文复制引用
邓显佑,薛艳,王嘉福,冉雪琴..贵州矮马鼠伤寒沙门氏菌的分离鉴定及其致病力研究[J].中国畜牧兽医,2016,43(10):2756-2761,6.基金项目
贵州省农业攻关项目(黔科合 NY 字2009-3068、黔科合 NY 字[2012]3009号) （黔科合 NY 字2009-3068、黔科合 NY 字[2012]3009号）
