中国畜牧兽医2016，Vol.43Issue(10)：2780-2785,6.DOI:10.16431/j.cnki.1671-7236.2016.10.040
牛源大肠杆菌O157∶H7的分离鉴定
Isolation and Identification of Escherichia coli O157∶H7 from Cattle
摘要
Abstract
The objective of this study was to investigate the infection and pathogenicity of E .coli O157∶H7 from 564 fecal samples in three parts of Xinjiang,such as Akesu,Yili and Tacheng. Suspected strains were detected by biochemical and PCR after enrichment by EC broth,being cho-sen by SMAC plate and screened by MUG test.On the basis of this,the isolated strains were used in mice attack drug test.The results showed that there were two strains of E .coli O157 ∶ H7 (named Y1 66 and Y226,respectively)were isolated from the collected samples of Yili,and the de-tection rate was 0.88%.In the mice attack drug test,the mice of Y1 66 and Y226 groups were all died within 48 h.In conclusion,two strains of E .coli O157∶H7 were isolated and identified with pathogenic from samples in Yili area,and no E .coli O157∶H7 were identified in the samples from Akesu and Tacheng.关键词
E.coliO157∶H7/分离鉴定/PCR/致病性Key words
E.coli O157∶H7/isolation and identification/PCR/pathogenicity分类
农业科技引用本文复制引用
王雨朦,张桃,丁浩,齐文静,何国文,苏战强,况玲..牛源大肠杆菌O157∶H7的分离鉴定[J].中国畜牧兽医,2016,43(10):2780-2785,6.基金项目
新疆维吾尔自治区自然科学基金资助项目(2014211A028) （2014211A028）
