中国药理学通报2016，Vol.32Issue(10)：1370-1376,7.DOI:10.3969/j.issn.1001-1978.2016.10.009
麦冬皂苷D通过降低自噬抑制血管紧张素Ⅱ诱导的心肌肥大
Ophiopogonin D attenuates angiotensin Ⅱ-induced myocardial hypertrophy by reducing autophagy
摘要
Abstract
Aim To study whether Ophiopogonin D has an effect inhibitory on myocardial hypertrophy induced by AngiotensinⅡand its possible mechanism. Methods Rat myocardial cell line H9 c2 were cultured in vitro. The effect of Ophiopogonin D on cell vitality was tested by;H9 c2 cells were treated with AngⅡ 1μmol ·L-1 after 24h to induce the cardiac hypertrophy,then it was co-treated with different concentrations of Ophio-pogonin D were added for 24h. After above,the total protein content was detected by BCA method;Quantita-tive real-time PCR ( qRT-PCR ) technique was used to examine the expression of marker genes BNP and β-MHC mRNA ,which representing the function of hear-ing; Western blot was used method to detect the ex-pression of autophagy protein LC3 B and high-through-put screening technology was emptoyed to verify it. In addi-tion, the changes of mitochondrial membrane po-tential in H9c2 myocardial cell were also examined. Results The cell viability results showed that H9 c-2 cells exposed to different concentrations of AngⅡ had no significant effect on vitality compared with the con-trol group after 24 h,but high concentrations of Ophio-pogonin D ( 50 ~100μmol · L-1 ) could obviously in-hibit the cell activity. Ot-her experimental results showed that myocardial cells treated with AngⅡ for 24h could cause myocardial hypertrophy,which appar-ently displayed the growth level of specific hypertrophic gene mRNA expression and the marked increase of the total protein expression. As hypertrophy was activated by AngⅡ, cells autophagy would be significantly en-hanced at the same time, more-over, the mitochondrial membrane potential would be reduced. But the effects of Ophiopogonin D could significantly reverse those pathological changes. Conclusion All above experi-mental results indicate that Ophiopogonin D can in-hibitmyocardial hypertrophy induced by AngⅡand pos-sibly plays a critical role in cardiovascular protection.关键词
麦冬皂苷D/血管紧张素Ⅱ/H9 c2细胞/心肌肥大/自噬/线粒体膜电位Key words
Ophiopogonin D/Angiotensin Ⅱ/H9 c2 cells/myocardial hypertrophy/autophagy/mitochondrial membrane potential分类
医药卫生引用本文复制引用
王远,王宇光,马增春,汤响林,梁乾德,谭洪玲,肖成荣,赵永红,高月..麦冬皂苷D通过降低自噬抑制血管紧张素Ⅱ诱导的心肌肥大[J].中国药理学通报,2016,32(10):1370-1376,7.基金项目
国家重点基础研究发展计划(973计划)资助项目( No 2012CB518402) （973计划）
