中国药理学通报2016，Vol.32Issue(10)：1377-1382,6.DOI:10.3969/j.issn.1001-1978.2016.10.010
降香水提物和挥发油对心肌缺血/再灌注损伤大鼠预防作用的代谢组学研究
Metabolomic study on preventive effect of Aqueous extract and Volatile oil of Dalbergia Odorifera on myocardial ischemia/reperfusion injury in rats
摘要
Abstract
Aim To investigate the changes of serum metabolism after the treatment of DOA and DOO on myocardial ischemia reperfusion ( MI/R ) injury in rats, and to explore the pathogenesis of MI/R injury and drug action mechanism. Method The serum samples of Sham group, Model group, DOA group and DOO group of rats were acquired, gas phase time of flight mass spectrometry ( GC-TOF-MS) was applied to analyze the metabolic profiles of the samples. After da-ta preprocessing, they were processed into SIMCA 14. 1 software for multivariate statistical analysis. Results By principal components analysis ( PCA ) , partial least squares analysis ( PLS-DA) and orthogonal partial least squares analysis ( OPLS-DA ) , the Model group and Sham group were obviously separated, the drug in-tervention group and Model group were separated and close to Sham group. The therapeutic effect of DOO and DOA on MI/R injury in rats was proved. The ex-perimental results identified 13 endogenous biomark-ers, which were related to the glucose metabolism,lipid metabolism and amino acid metabolism pathway. Con-clusion DOA and DOO may protect the MI/R injured rats by regulating the glucose metabolism, lipid metab-olism and amino acid metabolism pathway.关键词
代谢组学/气相飞行时间质谱/心肌缺血/再灌注损伤/降香水提物/降香挥发油/能量代谢Key words
metabonomics/GC-TOF-MS/MI/R inju-ry/DOA/DOO/energy metabolism分类
医药卫生引用本文复制引用
牟菲,段佳林,边海旭,蔺瑞,尚沛津,朱志会,爱东,奚苗苗..降香水提物和挥发油对心肌缺血/再灌注损伤大鼠预防作用的代谢组学研究[J].中国药理学通报,2016,32(10):1377-1382,6.基金项目
国家自然科学基金面上项目(No 81470174) （No 81470174）
