中国药理学通报2016，Vol.32Issue(10)：1383-1387,1388,6.DOI:10.3969/j.issn.1001-1978.2016.10.011
丹酚酸B保护大鼠缺血心肌与炎症小体NLRP3表达的相关性研究
Protective effects of salvianolic acid B on isoproterenol induced myocardial ischemic rats and its relation with NLRP3 expression
摘要
Abstract
Aim To evaluate the protective effects of salvianolic acid B on the ISO-induced myocardial is-chemic injury model of rats and the influence of regula-ting NLRP3 associated protein on myocardial ischemia. Method All rats were randomly divided into control group, model group and Sal B 5, 10, 15 mg · kg-1 groups. For 7 days, rats in Sal B groups were given by introperitoneal injection of 5, 10, 15 mg·kg-1 Sal B, rats in control group and model group were given the same volume of normal saline. Rats were subcutane-ously multi-point injected ISO ( 30 mg · kg-1 ) for 2 days on the fifth administrating day. The myocardial protective effect of Sal B was evaluated from electrocar-diogram( ECG), myocardial tissue pathological chan-ges, serum myocardial enzymes, oxidation index and inflammatory cytokine, myocardial tissue of NLRP3 related protein expression. Results Sal B could re-duce the degree of myocardial tissue necrosis and the infiltration of inflammatory cells, reduce T-wave values of ECG(P<0. 05 or P<0. 01). Compared with model group, CK values, GOT values and IL-1β values of rats in different dose groups were significantly lower, and MDA values and LDH values of rats in middle-and high-dose groups were significantly lower ( P<0. 05 or P<0. 01 ) . However, T-SOD values of rats middle-and high-dose groups were significantly higher ( P <0. 05 or P<0. 01). Meanwhile,the NLRP3, Caspase-1 and IL-1β protein level in myocardial tissue of the rats in different dose groups compared with model group had reduced ( P <0. 05 or P <0. 01 ) . Conclu-sion Sal B has protective effects on myocardial ische-mic rats, its mechanism may be related with inhibition of decreasing the expression of NLRP3 inflammasome associated protein, which can suppress the generation of inflammatory cytokines.关键词
丹酚酸B/NLRP3/异丙肾上腺素/心肌缺血/白细胞介素1-β/心肌酶Key words
salvianolic acid B/NLRP3/isoproterenol/myocardial ischemia/IL-1β/myocardial enzyme分类
医药卫生引用本文复制引用
王新宇,王曼,孙帅军,陈叶香,蒋宝平,许立..丹酚酸B保护大鼠缺血心肌与炎症小体NLRP3表达的相关性研究[J].中国药理学通报,2016,32(10):1383-1387,1388,6.基金项目
科技部“重大新药创制”重大科技专项( No 2011ZX09102-002-07) （ No 2011ZX09102-002-07）
江苏高校品牌专业建设工程资助项目( No PPZY2015A070) （ No PPZY2015A070）
