中国药理学通报2016，Vol.32Issue(10)：1388-1394,7.DOI:10.3969/j.issn.1001-1978.2016.10.012
马桑水提取物对烧伤创面感染常见3种耐药菌的抑制作用
Inhibition of Coriaria Sinica Maxim’ s extract on burn wound infections with common three kinds of resistant bacteria
摘要
Abstract
Aim To explore the inhibition of Sinica Maxim′s extract( CSME) on resistant infections of burn wounds,such as the methicillin-resistant staphylococcus aureus ( MRSA ) , resistant pseudomonas aeruginosa (RPA) and resistant escherichia coli(RECO). Meth-ods The resistant strains were cultured by MH agar plates. After resistance genes of quality control strains were extracted and appraised, such as mecA, mexB, merA, qacE△1-sull, tnpU/A and mexB, etc, and then,some projects of CSME were detected,such as the antibacterial spectrum, the minimum inhibitory con-centration(MIC), different concentrations of sensitive rate and inhibition curves, etc. Finally, these results were compared with the inhibitory effects of some anti-biotics to determine the sensitivity rates of CSME. Re-sults The MIC of CSME was 62. 5 ,125 ,250 g · L-1 respectively on the MESA, RPA and RECO. The inhi-bition rates of CSME appeared concentration-dependent on these three kinds of resistant bacteria,and the inhi-bition rates of the multi-concentration CSME on RECO were significantly lower than on MRSA and RPA ( P<0. 05). While in MIC,the resistance rates of MRSA on carbenicillin, cefazolin, erythromycin were significant-ly higher than those of CSME(P<0. 05); The inhibi-tion zones of CSME were significantly smaller than those of ceftriaxone, cefepime, imipenem, but greater than those of other antibiotics( P<0. 05 ); The inhibi-tion zones of CSME on RPA were significantly smaller than those of carbenicillin, and greater than those of other antibiotics ( P <0. 05 ) . The inhibition zones of CSME on RECO were significantly smaller than those of ceftriaxone,cefepime,imipenem,ciprofloxacin,nitro-furazone,and greater than those of other antibiotics ( P<0. 05 ) . Conclusions CSME has a significant inhi-bition on burn wound infection with these three kinds of resistant bacteria,such as MRSA,RPA and RECO. It is prompted that CSME could become one of the effective drugs to control burn wound infections with multi-re-sistant strains.关键词
马桑水提取物/烧伤感染/耐药菌/抑制作用/敏感率/抑菌曲线Key words
Coriaria Sinica Maxim’s extract( CSME)/burn wound infections/resistant/inhibition/sensitive rate/antibacterial curve分类
医药卫生引用本文复制引用
黄德斌,胡泽华,余昭芬,陈薛妃,黄谨..马桑水提取物对烧伤创面感染常见3种耐药菌的抑制作用[J].中国药理学通报,2016,32(10):1388-1394,7.基金项目
国家自然科学基金资助项目(No 81360654) （No 81360654）
