中国药理学通报2016，Vol.32Issue(10)：1395-1398,4.DOI:10.3969/j.issn.1001-1978.2016.10.013
基于荧光定量PCR评测热性中药成分对TRPV1通道功能的影响
Evaluation of regulational function of ingredients from hot herbs on TRPV1 channel based on 7900 PCR instrument
摘要
Abstract
Aim To further study the molecular mecha-nism of the herbs with hot nature on the regulational action on TRPV1 channel based on the 7900 Real-time PCR instrument. Methods 7900 PCR instrument was applied to detect the intracellular flurescence of TRPV1 channel in the dorsal root ganglions ( DRG ) neurons and the effect on the TRPV1 ’ s thermo-sensational functions of the selected 11 ingredients from hot herbs was explored. Results TRPV1 channels could be ac-tivated by gradually elevated temperature; the activa-tion process could be blocked by the TRPV1 specific blocking agent capsaizepine. Most of the ingredients from hot-nature herbs had the potential to up-regualate TRPV1 channel function. Conclusions The estab-lished TRPV1 channel detection system based on PCR instrument is suitable for the analysis of regulational functions of drugs on the heat-activated TRPV1 chan-nel;the functions of hot herbs may be related to the up-regualtional effects of its active ingredients on the TRPV1 channel which will further up-regulate energy metabolism.关键词
热性中药成分/背根神经节/TRPV1通道/7900 PCR仪/温觉感受功能/能量代谢Key words
ingredients from hot-nature herbs/dorsal root ganglion neurons/TRPV1 channels/7900 PCR in-strument/thermo-sensational function/energy metabo-lism分类
医药卫生引用本文复制引用
周海玉,戴丽,王德凤,戴逸飞,周炜炜,孟晶,隋峰,霍海如..基于荧光定量PCR评测热性中药成分对TRPV1通道功能的影响[J].中国药理学通报,2016,32(10):1395-1398,4.基金项目
国家自然科学基金面上项目( No 81274112,81373986,81473372) （ No 81274112,81373986,81473372）
国家自然科学基金青年项目( No 81403125,81403322) （ No 81403125,81403322）
北京市自然科学基金项目(No 7152106) （No 7152106）
