中国药理学通报2016，Vol.32Issue(10)：1399-1403,1404,6.DOI:10.3969/j.issn.1001-1978.2016.10.014
雷公藤甲素对人结肠癌HCT116细胞增殖、自噬和凋亡的影响
Effect of triptolide on human colorectal cancer HCT116 cell proliferation, autophagy and apoptosis
摘要
Abstract
Aim To investigate the effect of triptolide ( TP) on human colorectal cancer HCT116 cell prolif-eration and explore the potential mechanism of TP in treating colon cancer. Methods MTT assay was used for estimating the survival rates of HCT116 cells ex-posed to different concentrations and different duration time of TP. Western blot ( WB ) was used for testing the expression of LC3-Ⅱ. MDC staining was employed to detect cell autophagy. Flow cytometry ( FCM) was applied to test the effects of TP on cell apoptosis. Re-sults TP could inhibit cell proliferation in HCT116 cells. The expression of LC3-Ⅱwas enhanced with the increase of the concentration of TP after exposed to dif-ferent concentrations of TP for 48 h and the duration time of TP after exposed to 40 nmol·L-1 TP,and the number of acid autophagic vacuoles in HCT116 cells had increased, which was observed by fluorescence mi-croscope. The HCT116 cells apoptotic rates in TP group had decreased compared to control group and de-creased significantly compared to TP +3-MA group. The HCT116 cells apoptotic rates in TP group had in-creased compared to control group and increased signif-icantly compared to TP+RAPA group. Conclusion TP could inhibit the proliferation of human colon canc-er HCT116 cells and induce apoptosis in HCT116 cells, which indicates that synergy may exist between autophagy and apoptosis.关键词
雷公藤甲素/结肠癌/HCT116细胞/增殖/自噬/凋亡Key words
triptolide/colon cancer/HCT116 cell/proliferation/autophagy/apoptosis分类
医药卫生引用本文复制引用
赵林,吴鹏,章平贵,谢大泽,高典,周南进..雷公藤甲素对人结肠癌HCT116细胞增殖、自噬和凋亡的影响[J].中国药理学通报,2016,32(10):1399-1403,1404,6.基金项目
江西省自然科学基金资助项目(No 20142BAB205079) （No 20142BAB205079）
