中国药理学通报2016，Vol.32Issue(10)：1422-1426,1427,6.DOI:10.3969/j.issn.1001-1978.2016.10.018
虾原肌球蛋白及其IgE单克隆抗体建立I型过敏反应体内外模型
The in vitro and in vivo models induced by shrimp tropomyosin and monoclonal tropomyosin-specific murine IgE antibody
摘要
Abstract
Aim To develop the in vitro and in vivo models induced by shrimp tropomyosin( ST) and mono-clonal tropomyosin-specific murine IgE antibody ( anti-ST-IgE mAb) . Methods ST was purified from Metap-enaeusensis by an isoelectric precipitation method. The anti-ST-IgE mAb was obtained from hybridomas. After RBL-2 H3 cells were sensitized with anti-ST-IgE mAb and challenged with ST,β-hexosaminidase release was determined. Passive systemic anaphylaxis ( PSA ) was induced in mice and the rectal temperature was recor-ded after ST challenge within 30 min by a thermal probe. Results A significant increase ofβ-hexosamin-idase was observed in sensitized cells after ST chal-lenge. The average temperature drop after ST challenge was 1. 44℃ in PSA mice within 30 min. Conclusion The in vitro and in vivo models induced by ST and anti-ST-IgE mAb are established as an improvement of pres-ent models of type Ⅰ allergy.关键词
Ⅰ型过敏反应/原肌球蛋白/刀额新对虾/单克隆抗体/免疫球蛋白E/RBL-2H3细胞Key words
type Ⅰ allergy/tropomyosin/Metapenae-usensis/monoclonal antibody/IgE/RBL-2 H3 cells分类
医药卫生引用本文复制引用
方蕾,侯睿,费巧玲,高源,刘芬,蔡润兰,齐云..虾原肌球蛋白及其IgE单克隆抗体建立I型过敏反应体内外模型[J].中国药理学通报,2016,32(10):1422-1426,1427,6.基金项目
国家科技部“重大新药创制”科技重大专项( No 2014ZX 09201022-006) （ No 2014ZX 09201022-006）
北京市自然科学基金项目(No 7142112) （No 7142112）
