中国药理学通报2016，Vol.32Issue(10)：1463-1467,5.DOI:10.3969/j.issn.1001-1978.2016.10.025
“营卫通络方”对动脉粥样硬化早期颈动脉交感神经的调节作用
Influence of “Ying-nutrient and Wei-defense unblocking collaterals prescription”on carotid sympathetic nerve in early atherosclerosis
摘要
Abstract
Aim To observe the effect of“Ying-nutrient and Wei-defense unblocking collaterals prescription”on the carotid sympathetic nerve in early atherosclero-sis. Methods The rabbits were randomly divided into 6 groups:Control group, high-fat group, Complex mod-el group, GSTL-H group, GSTL-L group and ATO group. The control group was fed with common food-stuffs, High-fat group with high-fat diet to build early atherosclerosis model, and all the other groups with high-fat diet combined with carotid artery cannula to build early atherosclerosis carotid artery injury rabbit model. All groups were given corresponding medicines intragastrically once a day for 4 weeks. The GSTL-H and GSTL-L group was given Guishaotongluo ultrafine powder 0. 6 g·kg-1·d-1, 0. 3 g·kg-1·d-1, and the ATO group was given suspension of atorvastain so-lution 2. 5 mg · kg-1 · d-1 . Biochemical method was used to detect blood lipid change. HE staining was ap-plied to observe the pathological morphology of intima-media, The content of NE in the carotid arterial was detected by ELISA. The immunohistochemical staining was used to detect the protein expression of NGF, TH around the carotid arterial. The expression of NGF, TH in the carotid artery adventitia was detected by Western blot. Results Compared with normal group, the levels of TC,TG and LDL-C in high-fat group and complex model group were significantly increased( P<0. 01). The degree of the intimal hyperplasia,the con-tent of NE and sympathetic density ( NGF, TH protein expression and distribution ) of the cartoid artery in complex model group were heavier compared with those in high-fat group; the lipid levels, degree of the inti-mal hyperplasia and sympathetic density ( NGF, TH protein expression and distribution ) in the GSTL-H, GSTL-L group were milder in varying degrees compa-ring with those in the complex model group(P<0. 05, P<0. 01 ) . Conclusion “Ying-nutrient and Wei-de-fense unblocking collaterals prescription” can reduce rabbit carotid atherosclerosis, and the mechanism may be related to regulating the sympathetic nerve of arterial wall.关键词
动脉粥样硬化/血管外膜/营卫通络方,桂芍通络/阿托伐他汀/交感神经/神经调节Key words
atherosclerosis/adventitia/Ying-nutrient and Wei-defense unblocking collaterals prescription/Guishaotongluo/atorvastatin/sympathetic nerve/nerve modulation分类
医药卫生引用本文复制引用
尹玉洁,马柳一,贾振华,张军芳,王宏涛,单星阁,刘焕..“营卫通络方”对动脉粥样硬化早期颈动脉交感神经的调节作用[J].中国药理学通报,2016,32(10):1463-1467,5.基金项目
国家重点基础研究发展计划(973计划)资助项目( No 2012CB518606) （973计划）
河北省杰出青年科学基金项目( No H2015106063) （ No H2015106063）
