安徽农业科学2016,Vol.44Issue(26):1-3,3.
元蘑液体菌种的优化培养
Optimized Culture of Liquid Strains of Hohenbuehelia serotina
摘要
Abstract
Objective] To screen the medium formula of liquid strains of Hohenbuehelia serotina to optimize the culture process of liquid strains. [Method] By setting different liquid medium formulas,inoculation conditions (including strain age and amount of inoculum) and culture condi-tions (including rotation speed and culture time),culture conditions of liquid strains of H.serotina were optimized by myceliun biomass,density and shape of mycelium pellets.[Result] The optimal formula of the liquid medium of H.serotina strains are shown as follows:potato 200 g,straw powder 150 g,corn flour 10 g,glucose 20 g,peptone 3 g,yeast extract 2 g,and a piece of vitamin B1;the optimal age of strains was 5 d;the opti-mal amount of inoculum was 9%; the optimal rotation speed was 160 r/min;the optimal culture time was 7 d.[ Conclusion] By optimized cul-ture,the myceliun biomass,density and shape of mycelium pellets of H.serotina were greatly improved.关键词
元蘑/液体菌种/优化培养Key words
Hohenbuehelia serotina/Liquid seed/Optimized culture分类
农业科技引用本文复制引用
李延雷,陈炜东,滕素岩,高书成..元蘑液体菌种的优化培养[J].安徽农业科学,2016,44(26):1-3,3.基金项目
中央财政林业科技推广示范资金项目。 ()