安徽农业科学2016，Vol.44Issue(26)：4-6,22,4.
外源NO调控红松幼苗光合作用与抗氧化酶活性的浓度效应
Concentration Effects of Exogenous Nitric Oxide on Photosynthesis and Activity of Antioxidant Enzymes in Pinus koraiensis Seedlings
摘要
Abstract
Objective] To discuss the concentration effects of exogenous nitric(NO) oxide on photosynthesis and activity of antioxidant en-zymes in Pinus koraiensis seedlings.[Method] 3-year-old P.koraiensis seedlings were treated with sodium nitroprusside (SNP), a NO donor, at 5 different concentrations (0, 0.01, 0.10, 0.50 and 1.00 mmol/L).The contents of photosynthetic pigment, malondialdehyde (MDA) and hydrogen peroxide (H2O2), and the activity of antioxidant enzymes in the needles of P.koraiensis were determined.[Result] Net photo-synthetic rate (Pn), transpiration rate (Tr) and respiration rate (Rd) increased with the increase of SNP concentration, and reached a maxi-mum when SNP concentration was 0.10 or 0.50 mmol/L.Spraying 0.01 mmol/L SNP would significantly increase stomatal conductance (Gs), intercellular CO2 concentration (Ci) and photosynthetic pigment content, the activity of catalase (CAT) and peroxidase (POD).On the con-trary, the activity of superoxide dismutase (SOD), H2O2 and MDA content would decrease after the spraying of SNP.[Conclusion] Appropri-ate concentration of nitric oxide (NO) can significantly increase photosynthetic parameters, photosynthetic pigment content, CAT, APX and POD activity.Spraying SNP can decrease SOD activity, H2 O2 and MDA content.Exogenous NO can relieve the membrane lipid peroxidation of P.koraiensis seedlings to reduce the damage to the seedlings.关键词
红松/外源NO/生理指标Key words
Pinus koraiensis/Exogenous nitric oxide/Physiological indicators分类
农业科技引用本文复制引用
李德文,付金颖,魏晓雪,唐中华,祖元刚..外源NO调控红松幼苗光合作用与抗氧化酶活性的浓度效应[J].安徽农业科学,2016,44(26):4-6,22,4.基金项目
中央高校基本科研业务费专项（2572015CA03）；国家自然科学基金项目（31000133）；黑龙江省科学院青年创新基金项目。 （）
