安徽农业科学2016，Vol.44Issue(26)：10-12,3.
碱提麻竹竹秆多糖的相对分子质量测定及单糖组成分析
Determination of Relative Molecular Weight of Polysaccharides Extracting by Alkali Solution from the Pole of Dendrocalamus latiflo-rus and Analysis of Their Mono saccharide Composition
摘要
Abstract
Objective] To know more about bamboo.[Method] The polysaccharides in the pole of D.latiflorus were extracted by alkali solu-tion.The proteins were removed by Sevag agent from extraction.The polysaccharides in extraction were precipitated by alcohol of 95%, and then dialyzed by dialysis bag, separated by DEAE-52 cellulose column.Polysaccharides of DLP1 and DLP2 were obtained, and their relative molecular weights were measured and monosaccharide compositions were analyzed .[ Result] The relative molecular weights of polysaccharides DLP1 and DLP2 were detected to be 61 500 and 1 780 000 u.Their monosaccharide composition was determined by gas chromatography. DLP1 was composed of fucose (Fuc), rhamnose (Rha), alabinose (Ala), glucose (Glu) and galactose (Gal) at a quality ratio of 111.25∶17.81∶1∶12.43∶2.43.DLP2 was composed of fucose (Fuc), rhamnose (Rha), alabinose (Ala), glucose (Glu), galactose (Gal) and mannitol (Mnt) at a quality ratio of 198.22∶3.27∶9.16∶25.06∶10.96∶1.[Conclusion] The work laid a good foundation for studying on poly-saccharides of D.latiflorus, and provided scientific basis for further development of bamboo resource .关键词
麻竹多糖/碱提/相对分子质量/组成分析Key words
Polysaccharide in D.latiflorus/Alkali solution extraction/Relative molecular weight/Composition analysis分类
农业科技引用本文复制引用
潘文慧,阮期平,边清泉..碱提麻竹竹秆多糖的相对分子质量测定及单糖组成分析[J].安徽农业科学,2016,44(26):10-12,3.基金项目
四川省教育厅自然科学研究项目（13 ZB0115）；绵阳师范学院项目（MA2010005）。 （）
