安徽农业科学2016，Vol.44Issue(26)：16-19,4.
两步培养法测定厚垣孢普可尼亚菌产孢的最佳条件
Optimum Conditions for Sporulation of Pochonia chlamydosporia in Two-stage Cultivation
摘要
Abstract
Objective] To discuss the nutritional conditions for sporulation of Pochonia chlamydosporia.[Method] A two-stage cultivation meth-od was used to determine the effects of various nutritional and environmental conditions ( carbon sources,nitrogen sources,carbon-nitrogen molar ratio,and pH) on the sporulation of P.chlamydosporia PC021120-152.[Result] Chlamydospore production of PC021120-152 strains was the high-est when the initial carbon concentration was 3.00 g/L;the carbon-nitrogen molar ratio was 2.5∶1;sucrose/NaNO3 was as the carbon/nitrogen source,and the pH was 8.Conidiophore production of PC021120-152 strains was the highest when the initial carbon concentration was 3.00 g/L;the carbon-nitrogen molar ratio was 2.5∶1;D-fructose/NaNO3 was as the carbon/nitrogen source,and the pH was 5.0.[Conclusion] The re-search provides references for industrialization of biocontrol agents of P.chlamydosporia.关键词
厚垣孢普可尼亚菌/产孢/营养条件Key words
Pochonia chlamydosporia/Sporulation/Nutritional conditions分类
农业科技引用本文复制引用
石妍,肖顺,刘国坤,张绍升..两步培养法测定厚垣孢普可尼亚菌产孢的最佳条件[J].安徽农业科学,2016,44(26):16-19,4.基金项目
中国烟草总公司福建公司科技项目（闽烟2012［041］号）。 （）
