安徽农业科学2016，Vol.44Issue(26)：20-20,44,2.
菜豆花内生假单胞杆菌的分离与鉴定
The Isolation of an Endogenous Pseudomonas sp.from the Bean and Identification
摘要
Abstract
Objective] To isolate and identify an endogenous do minant (WBF1) from the bean flower.[Method] An endogenous dominant (WBF1) isolated from the bean flower was identified by gram staining, 16S rDNA and physiological and biochemical test.[Result] The gram staining result showed it was gram negative bacillus , and the 16S rDNA sequence analysis showed it belonged to Pseudomonas sp., Acinetobacter, Proteus.The physiological and biochemical characters corresponded with that of Pseudomonas sp.The bacterium grew in bean flower, and growth temperature was between 20 and 43 ℃.[Conclusion] The bacterium was an ideal candidate to be the engineered biocontrol host for the bean.关键词
菜豆/内生菌/染色/序列分析Key words
Bean/Endogenous bacteria/Staining/Sequence analysis分类
农业科技引用本文复制引用
董梦洁,左丹婷,胡玉,喻婉清,韩雪..菜豆花内生假单胞杆菌的分离与鉴定[J].安徽农业科学,2016,44(26):20-20,44,2.基金项目
湖北省教育厅指导性项目（ B2014080）；湖北省蔬菜豆类工程技术中心开放基金项目（201412）。 （）
