安徽农业科学2016，Vol.44Issue(26)：41-44,4.
L9（34）正交试验优化柑橘中橙皮苷的提取工艺
Extraction Process of Hesperidin in Citrus Optimized by L9 (34) Orthogonal Test
摘要
Abstract
Objective] In order to carry out the research on extraction and purification process of hesperidin in citrus peel, make full use of abundant citrus resources in Hanzhong and increase the added economical value.[Method] Taking the Hanzhong citrus peel as research mate-rial, hesperidin was extracted by alkali extraction and acid precipitation method.The single factor experiment was carried out with the kinds of alkali, alkali content, extraction temperature, extraction time and the ratio of material to liquid.Alkali dosage, extraction temperature, ex-traction time and solid-liquid ratio were studied by L9 (34 ) orthogonal test to optimize the extraction technology of hesperidin.The ultra per-formance liquid chromatography was used to test the optimization results .[ Result] The sequence for the factors affecting extraction and purifi-cation process of hesperidin in citrus peel was extraction time, alkali weight, extraction temperature and the ratio of material to liquid.When the process conditions were 0.08 g of Ca(OH)2 per gram citrus peel, extraction time 0.5 h, temperature 30 ℃, and the material and liquid ratio 1∶30 g/mL, the best extraction effect could be obtained.The yield rate of crude product was 4.5%.[Conclusion] The optimization pa-rameters and extraction process obtained in the test were stable, and the operation of selected equipment was simple and rapid, which had good application prospects.关键词
橙皮苷/碱浸酸沉/提取/工艺/纯化Key words
Hesperidin/Alkali extraction-acid precipitation/Extraction/Process/Purification分类
农业科技引用本文复制引用
杜欢欢,张涵,景娴,孟怡璠,江海,徐皓..L9（34）正交试验优化柑橘中橙皮苷的提取工艺[J].安徽农业科学,2016,44(26):41-44,4.基金项目
陕西省教育厅项目（14JS018）。 （）
