安徽农业科学2016,Vol.44Issue(27):89-92,4.
2种常用食用菌所含多糖化合物复配的体外抗氧化活性研究
Antioxidant Activity of the Compound Polysaccharides of Two Common Edible Fungus
倪焱 1张雄飞 1王仲1
作者信息
- 1. 武汉华夏理工学院生物与制药工程学院,湖北武汉430223
- 折叠
摘要
Abstract
Objective] To study the antioxidant activity of polysaccharides of Flammulina velutipes and Pleurotus ostreatus compound in vitro,to explore the feasibility and effectiveness of compound polysaccharides with hydroxyl radical ( OH・ ) and superoxide anion ( O2-・ ) as evaluation indexes.[Method] With F.velutipes and P.ostreatus as the raw materials,extraction condition of polysaccharides was optimized.Antioxidant activ-ity in vitro was researched to measure the clearance rates of OH・ and O2-・ ,to study the scavenging activity of free radical of compound polysac-charides,and to compare it with single polysaccharide.[Result] The optimal extraction conditions of polysaccharide were 30 min ultrasonic wave, 90 ℃ extraction temperature of hot water.Under this condition,the extraction rate of F.velutipes was 1.25%and the purity of polysaccharides was 84.3%.The extraction rate of P.ostreatus was 1.46%,and the purity of polysaccharides was 76.4%.The ability of compound polysaccharides to remove OH・ rose to higher concentration.Under F.velutipes∶P.ostreatus =5∶1,compound polysaccharides reflected good cooperativity,which was superior to single polysaccharide.The ability of compound polysaccharides to remove O2-enhanced in higher concentration.Especially under low concentration and F.velutipes∶P.ostreatus=3∶1,compound polysaccharides showed better cooperativity.[Conclusion] The compound of edi-ble fungus polysaccharides can effectively improve its antioxidant activity.关键词
金针菇/平菇/多糖/抗氧化活性/复配Key words
Flammulina velutipes/Pleurotus ostreatus Polysaccharides/Antioxidant activity/Compound分类
农业科技引用本文复制引用
倪焱,张雄飞,王仲..2种常用食用菌所含多糖化合物复配的体外抗氧化活性研究[J].安徽农业科学,2016,44(27):89-92,4.