安徽农业科学2016，Vol.44Issue(27)：127-130,4.
泰山螭霖鱼线粒体COⅠ基因序列的遗传多样性分析
Study on Genetic Diversity of Varicorhinus macrolepis Based on COⅠ Gene Sequences
摘要
Abstract
Objective] Through cloning COⅠ gene sequence of Varicorhinus macrolepis, its evolutionary relationship with other genus fish in Cyprinidae was studied, the classification status of V.macrolepis was determined.[ Method] 30 V.macrolepis were used as experimental mate-rials, mitochondrial cytochrome oxidase subunitⅠ( COⅠ) gene fragment was amplified using PCR technique and aligned of sequence, genetic diversity of V.macrolepis was detected,and genetic distance between different haplotypes of V.macrolepi and 5 genus of Cyprinidae in Gen-Bank was calculated.Clustering analysis was used to build NJ and UPGMA phylogenetic tree.[ Result] The results showed that the COⅠgene fragment PCR products of 30 V.macrolepis were about 1 600 bp, and no insertion/deletionwas observed, 1 493 bp nucleotide sequences were determined after being purified and excluded the primer sequence.There were 6 haplotypes, and 6 polymorphic sites were detected, including 4 transition sites and 2 transversion sites.Haplotype diversity (Hd) of V.macrolepis was 0.460, nucleotide diversity (Pi) was 0.000 78, av-erage number of nucleotide differences ( K) was 1.163.Genetic distance and NJ UPGMA phylogenetic trees showed that the sequences of V.macrolepis and O.macrolepis are the same.The genetic relationship between Varicorhinus and Acrossocheilus was the closest, while the ge-netic relationship between Varicorhinus and Spinibarbus, Sinilabeo,Sinocyclocheilus were far.[ Conclusion] The study can provide molecular biology basis for management, protection and genetic breeding of germplasm resources.关键词
泰山螭霖鱼/COⅠ/遗传多样性Key words
Varicorhinus macrolepis/COⅠ Gene/Genetic diversity分类
农业科技引用本文复制引用
杨玲,巩俊霞,付佩胜,李娴,张金路..泰山螭霖鱼线粒体COⅠ基因序列的遗传多样性分析[J].安徽农业科学,2016,44(27):127-130,4.基金项目
山东省农业良种工程项目“水产经济生物种质资源收集保护与评价”子课题三“淡水水产经济生物种质资源收集、保护与评价”；山东省现代农业产业体系鱼类创新团队项目（SDAIT－12－04）。 （）
