滨州医学院学报2016，Vol.39Issue(5)：330-332,3.
Pyk2在结直肠癌中作用机制的初步研究
The preliminary study on the mechanism of Pyk2 in colorectal cancer
摘要
Abstract
Objective To explore the mechanism of proline‐rich protein tyrosine kinase‐2 (Pyk2) in human colorectal carcino‐ma .Methods Recombined plasmids pcDNA4 .1/Zeocins constructed with sense and antisense Pyk2 cDNA were stably trans‐fected into sw480 cells ,respectively .The changes in apoptosis and cell cycle were determined by Flow cytometry (FCM) .Re‐sults The apoptosis rate of SW480 cells transfected with Pyk2 cDNA plasmid was significantly higher than the control group , the number of cells distributed in s stage decreased significantly ;while the apoptosis rate of cells transfected with antisense Pyk2 plasmid was significantly lower than that of the control group ,and the number of cells distributed in s stage increased sig‐nificantly .Conclusion Pyk2 could up‐regulate the apoptosis level of sw 480 cells ,while lower its proliferation .关键词
结直肠癌/蛋白酪氨酸激酶2/增殖/凋亡/转染Key words
colorectal carcinoma/Pyk2/proliferation/apoptosis/transfection分类
医药卫生引用本文复制引用
张宗耐,常迎彬..Pyk2在结直肠癌中作用机制的初步研究[J].滨州医学院学报,2016,39(5):330-332,3.
