草业学报2016，Vol.25Issue(10)：56-65,10.DOI:10.11686/cyxb2015586
库布齐沙地两种植被恢复类型根际土壤微生物群落功能多样性研究
Rhizosphere microbial functional diversity affected by vegetation restoration in the Hobq Sand Land,Inner Mongolia,China
摘要
Abstract
Soil microbial functional diversity is an important indicator of the condition and function of soil micro-bial communities and is also a useful indicator in the process of ecological restoration.We sought to study the effects of different vegetation restoration techniques on microbial functional diversity during ecological restora-tion in the Hobq Sand Land.Two types of vegetation restoration were compared;natural restoration of Arte-misia ordosica and reintroduction of Caragana intermedia ,which began in 1995,using a Biolog micro-plate technique with untreated sandy soil as the control.The results demonstrated that:1 )compared to untreated land,vegetation restoration resulted in increases in the rate of soil microbial carbon source utilization and com-munity metabolic diversity.Between the two restoration types,both soil microbial carbon source utilization rate and community metabolic diversity in the rhizosphere and non-rhizosphere soils were higher in sites re-stored with A.ordosica than in sites restored with C.intermedia.2)The carbon sources predominantly used by soil microbes in untreated soil were amino acids,amines,and phenolic acids;these were replaced by carbo-hydrates,carboxylic acids,and polymers as vegetation recovered;these differences were significant.3)Princi-pal component analysis demonstrated that there were differences in soil microbial metabolic activity among dif-ferent vegetation restoration systems,in particular,carbohydrates.4)Soil nitrogen (N)was strongly correla-ted with microbial functional diversity.Redundancy analysis (RDA)of microbial communities constrained by different soil factors showed that soil organic matter,total N,available K,available N,pH,and total P were important factors determining the rate of metabolism of carbon substrates.It was concluded that natural resto-ration of the A.ordosica community was better at fostering soil microbial communities capable of using differ-ent carbon sources with enhanced microbial metabolic activity than reintroduction of the C.intermedia and the untreated approach.关键词
植被恢复/土壤微生物/Biolog/功能多样性Key words
vegetation restoration/soil microorganism/Biolog/functional diversity引用本文复制引用
戴雅婷,侯向阳,闫志坚,解继红,吴洪新..库布齐沙地两种植被恢复类型根际土壤微生物群落功能多样性研究[J].草业学报,2016,25(10):56-65,10.基金项目
中央级公益性科研院所基本科研业务费专项资金项目（1610332014026）和国家重点基础研究发展计划（973计划）（2014CB138806）资助。 （）
