福建农业学报2016,Vol.31Issue(8):820-825,6.DOI:10.19303/j.issn.1008-0384.2016.08.006
天宝蕉HOS1基因启动子克隆及生物信息学分析
Cloning and Bioinformatic Analysis of HOS1 Promoter from the ‘Tianbao’Banana (Musa acuminata. AAA group)
摘要
Abstract
In this experiment,HOS1 promoter was cloned successfully from the ‘Tianbao’banana,and was further bioinformatically predicted. The analysis results showed that the sequence similarity of HOS1 promoter was 92.43% between the Tianbao banana and the wild banana in Malaysia,and the Tianbao banana promoter contained 35 kinds of cis-elements,including a variety of types of phytohormone response cis-elements and cis-elements concerning abiotic stress,which suggested that there be a close relationship with cold stress response in Tianbao banana. Tianbao banana HOS1 promoter contained two CpG islands,which suggested it should also have a close relationship between cold stress response and methylation in Tianbao banana.关键词
天宝蕉Musaacuminata,AAA group/HOS1/启动子/克隆/生物信息学分析Key words
‘Tianbao’banana (Musa spp.,AAA group)/HOS1/promoter/cloning/bioinformatic analysis分类
农业科技引用本文复制引用
刘炜婳,林争春,冯新,赖钟雄..天宝蕉HOS1基因启动子克隆及生物信息学分析[J].福建农业学报,2016,31(8):820-825,6.基金项目
福建省重大科技专项(2015NZ0002-1) (2015NZ0002-1)
国家香蕉产业技术体系专项(CARS-32-11) (CARS-32-11)