福建农业学报2016，Vol.31Issue(8)：826-832,7.DOI:10.19303/j.issn.1008-0384.2016.08.007
铁观音茶树RING型E3泛素连接酶基因克隆及其干旱胁迫下的表达分析
Cloning and Expression Analysis of Ring-type E3 Ubiquitin Ligase Gene in Camellia sinensis cv. Tieguanyin Under Drought Stress
摘要
Abstract
The E3 ubiquitin ligase gene from Camellia sinensis cv.Tieguanyin tea plants were cloned and subj ected to a bioinformatics analysis.Under varied drought conditions on the plants,the quantitative expressions of the gene was determined using qPCR.The gene sequence (GenBank accession number,KR819177)was found to be 1 138 bp long containing an open reading frame (ORF)of 810 bp and encoding 269 amino acids.The bioinformatics revealed that the ligase gene did not have a transmembrane or signal peptide but multiple phosphorylation sites,and that its subcellular cells located in the chloroplasts.Its nucleotide and deduced amino acid sequences showed 51%,50%, 50%,50%,and 49% homology with the E3 ubiquitin ligase genes from Nicotiana sylvestris,Camelina sativa, Vitis vinifera,Tarenaya hassleriana,and Brassica rapa,respectively;while its conserved functional domains related to the translated protein sequences had a RING-finger structure.Consequently,it was preliminarily identified as the E3 ubiquitin ligase gene of C.sinensis cv.Tieguanyin.Subsequently,the qPCR analysis indicated that the transcript of this gene,when the tea plant was under varied drought stresses,was significantly more up-regulated than control.Hence,it was concluded that the previously identified RING-type E3 ubiquitin ligase gene found in C.sinensis cv.Tieguanyin indeed involved in the drought response mechanism of the plant.关键词
铁观音/RING型 E3 泛素连接酶/基因克隆/干旱胁迫/qPCRKey words
Camellia sinensis cv.tieguanyin/RING-type E3 ubiquitin ligase/gene cloning/drought stress/qPCR分类
农业科技引用本文复制引用
常笑君,郭玉琼,王仲,赵姗姗,朱晨,林玉玲,赖钟雄..铁观音茶树RING型E3泛素连接酶基因克隆及其干旱胁迫下的表达分析[J].福建农业学报,2016,31(8):826-832,7.基金项目
福建省自然科学基金(2013J01085) （2013J01085）
福建省重大科技专项(2015NZ0002-1) （2015NZ0002-1）
福建省教育厅项目(JA15143) （JA15143）
