福建农业学报2016，Vol.31Issue(8)：858-862,5.DOI:10.19303/j.issn.1008-0384.2016.08.013
番茄青枯病植物疫苗菌株FJAT-1458的培养条件优化及发酵过程状态参数研究
Optimization of Culture Conditionsand Analysis of Fermentation Process for Plant Vaccine-producing Bacterium,FJAT-1 4 5 8
摘要
Abstract
Conditions to culture FJAT-1458,a bacterium that produces plant vaccine against the tomato bacterial wilt caused by Ralstonia solanacearum,were optimized by asingle factor experiment.They were determined to be the use of an SPA medium containing peptone 5 g·L-1 ,sugar 20 g·L-1 ,KH2 PO4 0.5 g·L-1 ,and MgSO4 0.25 g·L-1 at 30℃ with 35 mL of broth in a 250 mL shaking flask.Subsequently,in a 50 L fermentation tank,the fermentation was carried out and the process monitored.During the fermentation,the cell count on FJAT-1458 increased initially and followed by a gradual decline with a 4-stage growth period that included the adaptive (0-6 h),logarithmic (6-36 h),stationary (36-60 h),and decline phases (after 72 h).In addition,it was observed that (a)the pathogenicity indicator,attenuation index,of FJAT-1458 ranged from 0.832 to 0.855 showing no significant difference (P>0.05)throughout the entire process;(b)the pH of the fermentation broth increased from 7.23 to 8.78;(c)morphologically,the bacteria appeared to be (1.0-1.5)μm× (0.5-0.8)μm rods with a darkening color as the process proceeded;(d)the cellular secretion from the bacteria increased with time;and,(e) some of the bacterial cells deformed and lysed near the end of fermentation.关键词
番茄青枯病/青雷尔氏菌/植物疫苗/培养条件优化Key words
tomato bacterial wilt/Ralstonia solanacearum/plant vaccine/culture condition optimization分类
农业科技引用本文复制引用
郑雪芳,朱育菁,刘波,葛慈斌..番茄青枯病植物疫苗菌株FJAT-1458的培养条件优化及发酵过程状态参数研究[J].福建农业学报,2016,31(8):858-862,5.基金项目
国家公益性行业(农业)科研专项(201303015) （农业）
福建省自然科学基金项目(2015J01103) （2015J01103）
福建省科技计划项目---省属公益类科研院所基本科研专项(2014R1018-8) （2014R1018-8）
