福建农业学报2016，Vol.31Issue(8)：869-875,7.DOI:10.19303/j.issn.1008-0384.2016.08.015
高产过氧化氢酶菌株的鉴定与产酶条件
Identification and Optimized Fermentation of High Catalase-producing Serratia marcescens,FZSF0 2
摘要
Abstract
To search for a high catalase-producing bacteria strain,field samples and lab collections were screened. FZSF02 was initially selected and subjected to morphological examinations and 16S rRNA sequencing.It was identified to be a strain of Serratiamarcescens.Subsequently,various carbon and nitrogen sources,mineral salts, and fermentation conditions to maximize the catalase production of the bacteria were experimented and optimized.It was found that a medium consisting of 1% soy peptone,0.5% lactose,and 0.2% NaCl applied for the fermentation at 31℃ with a constant shaking at 180rpm for 48 h could yield the enzyme at the highest level of 6 380 U·mL-1 among all tested conditions.The yield was 7.4 times of what was without the optimization.The peak activity of the obtained crude catalase was determined to reach at 60℃ and pH 8.0.The enzyme could potentially be applied for treating hydrogen peroxide pollutionin waste water effluent.关键词
黏质沙雷氏菌/过氧化氢酶/产酶条件Key words
Serratiamarcescens/catalase/fermentation conditions分类
生物科学引用本文复制引用
田燕丹,贾宪波,林新坚,邱宏端,陈济琛..高产过氧化氢酶菌株的鉴定与产酶条件[J].福建农业学报,2016,31(8):869-875,7.基金项目
国家公益性农业科研专项(201303094-05) （201303094-05）
福建省财政厅社会公益研究项目(2060302) （2060302）
福建省科技计划项目---省属公益类科研院所基本科研专项(2009R10002-2) （2009R10002-2）
星火计划重点项目(2014S0068) （2014S0068）
