福建农业学报2016，Vol.31Issue(8)：881-885,5.DOI:10.19303/j.issn.1008-0384.2016.08.017
高效液相色谱法检测土壤中氧化乐果残留
HPLC Determination of Omethoate Content in Soil
摘要
Abstract
Testing conditions for the omethoate determination byHPLC were optimized to be:Inertsil QDS-SP (4.6∗250μm)at 25℃,an injection of 20μL methanol/water (V/V=10/90)as mobile phase ata flow rate of 1.0 mL·min-1 ,and the wave length of 210 nm for ultraviolet detection.A high recovery rate was achieved by using anextraction column with acetone/acetonitrile (V/V=1/3 )as the eluting agent.A linear calibration curve for the omethoate concentrations in the range of 0.1-10μg·mL-1 (R2=0.9992)with a minimal detection limit of 0.1μg ·mL-1 was established.Acetone and ethyl acetate were used as the solvents in the oscillation extraction.The recovery rate of omethoate in the soil samples ranged from 70% to 120% with a relative deviation of 2.08%-8.08%,which was within the requirements for pesticide residue analysis.It appeared that the newly developed HPLC methodology was appropriate for detecting omethoate residues in soil.关键词
高效液相色谱/氧化乐果/土壤/有机磷农药Key words
HPLC/omethoate/soil/organophosphorus pesticides分类
资源环境引用本文复制引用
李若云,张娇..高效液相色谱法检测土壤中氧化乐果残留[J].福建农业学报,2016,31(8):881-885,5.基金项目
国家自然科学基金项目(41301552、41201507) （41301552、41201507）
青岛农业大学应用型人才培养特色名校建设工程课程建设项目(XDSJP2013037) （XDSJP2013037）
