广西植物2016，Vol.36Issue(10)：1220-1224,5.DOI:10.11931/guihaia.gxzw201601026
大豆PM34基因生物信息学预测及表达分析
Bioinformatics and the expression pattern analysis of soybean PM34 gene
摘要
Abstract
In this paper, the structure and tissue-specificity of the soybean seeds mature protein gene ( PM34 ) were studied, which could become the the foundations for further research the function of PM34 gene in soybean or the other plants. The sequence of soybean(Jidou2) PM34 gene was amplified by RT-PCR with the total RNA of soybean leaf as templet. The structure of soybean PM34 gene was predicted by bioinformatics. The expression pattern of soybean PM34 gene in soybean organs was detected by real-time quantitative PCR (qRT-PCR). The results showed that the molecular weight of the protein was 31.7 kDa, the isoelectric point was 6.60, and belonging to the hydrophilic protein. There was absent transmembrane domain and singal peptide. The secondary structure was comprosed of 12. 97% alpha helix, 41.30% coil and 45.73% extended strand. The protein tertiary structure prediction showed that the homologous model template was 3 ijr. 1. A, which was a kind of short chain dehydrogenase/reductase and the homology was 54%. The evo-lutionary relationship demonstrated that soybean had relatively closer relationship with Vigna radiata and Medicago trun-catula than others. In addtion, the result showed that there was a little activity in roots, stems, leaves and flowers, but there was the highest activity in seeds, especially in mature seeds.关键词
大豆/PM34基因/克隆/生物信息学/qRT-PCRKey words
soybean/PM34 gene/cloning/bioinformaics/qRT-PCR分类
农业科技引用本文复制引用
赵艳,翟莹,宫国强,王曼,赵阳..大豆PM34基因生物信息学预测及表达分析[J].广西植物,2016,36(10):1220-1224,5.基金项目
黑龙江省自然科学基金(C201458)；黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT 2016090)；国家自然科学基金(31301335)[Supported by the Natural Science Foundation of Heilongjiang Province (C201458)； Training Plan of Young Creative Talents of Average Four-Year College of Heilongjiang (UNPYSCT 2016090)；the National Nature Science Foundation of China (31301335)]。 （C201458）
