广西植物2016，Vol.36Issue(10)：1238-1244,1274,8.DOI:10.11931/guihaia.gxzw201512019
不同抗生素对金发草愈伤组织生长分化的影响
Effects of different antibiotics on callus differentiation and growth of Pogonatherum paniceum
摘要
Abstract
In the study, Pogonatherum paniceum calluses were selected as the experimental materials, and the effects of different kinds of antibiotics ( kanamycin, hygromycin, ceftiofur sodium and ampicillin) and its concentrations to the growth and differentiations of P. paniceum calluses were compared and analysed in order to find out the suitable resist-ance selection agent and antibacterial agent in genetic transformation system of P. paniceum, respectively. The results were as follows:(1) P. paniceum callus had a strong sensitivity to kanamycin and the differentiation rate significantly decreased as the concentration of kanamycin increased (P=0.01). When the concentration of kanamycin was 10 mg· L-1 , the growth and differentiation of P. paniceum callus were significantly suppressed and differentiated a large numberof whiten seedlings, but the differentiation rate was still at 36.56%. When the concentration of kanamycin was 15 mg· L-1 , differentiation rate of P. paniceum callus was at 11. 94%, and only part of calluses were differentiated into green seedlings. However, when the concentration of kanamycin was 20 mg · L-1 , P. paniceum callus almost turn brown or dead, and the differentiation rate was only at 2.26%. Hence, 15 mg·L-1 of kanamycin was fit for serveing as the resist-ance selection agent in genetic transformation system of P. paniceum. (2) P. paniceum callus had a less strong sensitivity to hygromycin than kanamycin. The effects of hygromycin on differentiation rate of P. paniceum callus was very small, but the toxicity was big, so hygromycin was not suitable as the resistance selection agent in genetic transformation system of P. paniceum. (3) 300 mg·L-1 of ceftiofur sodium and ampicillin had a little effect on the growth and differentiation of P. paniceum callus, and all were able to inhibit the bacterials effectively. Morever, the inhibition of little higher level of ampicillin to the growth and differentiation of P. paniceum callus was less obvious. Therefore, 300 mg·L-1 of ceftiofur sodium and little higher level of ampicillin could be used as the antibacterial agent in genetic transformation system of P. paniceum as well. This study found out the suitable kinds and concentration of antibiotics served as the resistance se-lection agent and the antibacterial agent in Agrobacterium tumefaciens mediated genetic transformation system of P. pani-ceum, respectively, which provides the reference for studing genetic improvement and functional genes of P. paniceum.关键词
金发草/愈伤组织/抗生素/遗传转化/抗性筛选剂/抑菌剂Key words
Pogonatherum paniceum/callus/antibiotics/genetic transformation/resistance selection agent/antibac-terial agent分类
农业科技引用本文复制引用
耿天龙,李佛生,于敏,罗枫雪,唐琳,王胜华..不同抗生素对金发草愈伤组织生长分化的影响[J].广西植物,2016,36(10):1238-1244,1274,8.基金项目
国家自然科学基金(31270360)[Supported by the National Natural Science Foundation of China (31270360)]。 （31270360）
