广西植物2016,Vol.36Issue(10):1253-1258,6.DOI:10.11931/guihaia.gxzw201509008
姜科植物的FTIR鉴别分类研究
Discrimination of Zingiberaceae using FTIR spectroscopy
摘要
Abstract
We measured ginger Zingiberaceae subfamily six species of two genera and 29 species of plants spectrum measurement with Fourier transform infrared spectroscopy (FTIR), and analysed and compared different genra of Zin-giberaceae and different species of the same genus in the infrared spectrum picture. The combined with clustering analy-sis of. Infrared spectrum chart peak positions were basically the same, and the chemical composition were roughly the same. They were composed of carbohydrate, protein, lipid and other biological molecular vibration spectrum band. From 1 800 to 1 000 cm-1 for second derivative conduction, the difference were mainly concentrated in 1 750-1 400, 1 200-1 000 cm-1 region, the second derivative could increase the difference of spectral peaks, and improve the resolution of the spectrum. The 1 800-1 000 cm-1 the second derivative processing, and the differences of the 1 750-1 400, 1 200-1 000 cm-1 were compared. The absorbance ratios A1516/A3424 and A1449/A3424 were used to reflect the relative content of gingerols and diarylheptanoids, and then the data were analyzed and clustered. The results showed that relative contentsof the main chemical constituents of the plant of gingerols and diarylheptanoids compounds in family and species were quite different;the relative contents were significantly different in different gera of the same group, but were similar in different species of the same genus plants. The chemical composition of the sugars in the plant of the ginger family were composed of many types of compounds, but mainly monosaccharides and polysaccharides. At the level of the family, the classification of six families and two genera of the family was in agreement with the results of the traditional classifica-tion, and from the point of view of the spectrum. It is suggested that the group level classification of B. longiflora should take the time, energy, and more information and further research in many aspects. It can be known that the Fourier transform infrared spectroscopy combined with clustering analysis method can be applied to the classification of Zingiber-aceae. This study provides an academic value and a meaningful reference for the classificeation of ginger family.关键词
傅里叶变换红外光谱/聚类分析/姜科植物/鉴别分类/族属Key words
Fourier transform infrared spectroscopy/system cluster/Zingiberaceae/discrimination/clan分类
农业科技引用本文复制引用
刘艳,司民真,李家旺,李伦,张德清..姜科植物的FTIR鉴别分类研究[J].广西植物,2016,36(10):1253-1258,6.基金项目
国家自然科学基金(13064001,10864001)[Supported by the National Natural Science Fundation of China(13064001,10864001)]。 (13064001,10864001)