湖南中医药大学学报2016，Vol.36Issue(10)：11-17,7.DOI:10.3969/j.issn.1674-070X.2016.10.004
抗纤灵Ⅰ方对小鼠血吸虫病肝纤维化的干预及机制探讨
The Intervention Effects and Mechanisms of KangXianLing PrescriptionⅠon Schistosomiasis Liver Fibrosis in Mice
摘要
Abstract
Objective To explore the intervention effects of KangXianLing prescriptionⅠ(KXLPI) on schistosomiasis liver fibrosis in mice, elucidate the possible molecular mechanisms of KXLPⅠ anti hepatic fibrosis, and provide experimental basis for clinical application of KXLPⅠ. Methods Kunming mice were infected by Sj cercariae in order to establish the animal model with hepatic fibrosis. All the model mice with hepatic fibrosis were treated by praziquantel (PZQ) and randomly divided into 9 groups, including KXLPⅠlow dose group (L-KXLPⅠ), medium dose group (M-KXLPⅠ) and high dose group (H-KXLP I), KXLPⅠ's modified prescription based on Salvia miltiorrhiza (KXLPⅠ-Sm) low dose group (L-KXLPⅠ-Sm), medium dose group (M-KXLPⅠ-Sm) and high dose group (H-KXLPⅠ-Sm), Colchicin treatment group (CTG), distilled water group (DWG)and model group (MG), and the mice which were not infected by Sj cercariae are used as normal control group (NG). After intragastric administration for 8 weeks, the livers of the mice were collected and the pathologic changes of hepatic fibrosis were observed by HE. The mean granuloma areas induced by eggs were detected, and pathological changes of hepatic fibrosis were observed. The expression levels of collagen Ⅰ, Ⅲ, matrix-metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were detected by immunohistochemical staining, the differences of proteins were compared. Results Compared with MG and NG, the mean granuloma areas in H- KXLPⅠand H-KXLPⅠ-Sm significantly decreased, (P<0.05). Immunohistochemical staining results showed that the expression levels of collagen Ⅰ, Ⅲ and TIMP-1 in M-KXLPⅠ, H- KXLPⅠ, M-KXLPⅠ-Sm and H-KXLPⅠ-Sm were significantly lower than that of MD group (P<0.05), while the expression levels of MMP-1 significantly higher than that of MD (P<0.05), there is no difference between KXLPⅠand KXLPⅠ-Sm groups (P>0.05). Conclusion KXLPⅠand KXLPⅠ-Sm showed good preventive effects on experimental schistosomiasis liver fibrosis mice, but there was no difference on curative effect, and Salvia miltiorrhiza could not enhance the effect of KXLPⅠanti hepatic fibrosis. Meanwhile, KXLPⅠcould inhibit the expressions of collagenⅠ, Ⅲ and TIMP-1 in liver tissues and increase the expression of MMP-1.关键词
抗纤灵Ⅰ方/血吸虫病/肝纤维化/胶原蛋白Ⅰ/胶原蛋白Ⅲ/MMP-1蛋白酶/TIMP蛋白/丹参Key words
KangXianLing Prescription I/schistosomiasis/hepatic fibrosis/collagen Ⅰ/collagen Ⅲ/matrix-metal loproteinase-1/tissue inhibitor of metalloproteinase-1/Salvia miltiorrhiza分类
医药卫生引用本文复制引用
刘西霞,黄政德,卢芳国,高强,赖娟,芦俊,向琴,刘水平,杨胜辉..抗纤灵Ⅰ方对小鼠血吸虫病肝纤维化的干预及机制探讨[J].湖南中医药大学学报,2016,36(10):11-17,7.基金项目
国家自然基金项目（81373576）；湖南省自然科学基金项目（11JJ3106）；湖南省中医药管理局重点项目（201209）；湖南省卫生厅基金项目（B2014-043）；湖南省高校感染性疾病中医药防治研究科技创新团队开放基金项目（Grxjb-3）；湖南省教育厅基金项目（15C0147）。 （）
