湖南中医药大学学报2016，Vol.36Issue(10)：22-26,5.DOI:10.3969/j.issn.1674-070X.2016.10.006
金匮肾气丸对单侧输尿管梗阻大鼠肾组织p-STAT3蛋白的影响
Effect of Jingui Shenqi Pill on the Expression of p-STAT3 in Unilateral Ureteral Obstruction Rats
摘要
Abstract
Objective To investigate the effect of Jingui Shenqi pill on unilateral ureteral obstruction (UUO) rats with renal interstitial fibrosis and the expression of p-STAT3 protein. Methods 30 male SD rats were randomly divided into sham operation group, UUO model group, low dose of Jingui Shenqi pill group, high dose of Jinguishenqi pill group, enalapril treatment group as positive control. After intragastric administration on the 14 days, the rats were sacrificed. The obstructive kidney tissues were stained with HE and Masson to observe the pathologic changes. The protein or mRNA expression of type I and III collagen in renal tissue was detected by immunohistochemistry staining or Real-time PCR. The phosphorylation of signal transducer and activator of transcription 3 (STAT3) in renal tissue was detected by Western blot. Results Compared with UUO model group, after treatment with Jingui Shenqi pill, the renal interstitial damage index and collagen positive area were decreased, the expression of type I and type III collagen protein and mRNA were also decreased and the phosphorylation of STAT3 protein was inhibited (P<0.05). Conclusion Jingui Shenqi pill can obviously improve renal interstitial injury and reduce interstitial collagen deposition in UUO rats which may be related to the inhibition of STAT3 phosphorylation.关键词
金匮肾气丸/肾纤维化/单侧输尿管梗阻/大鼠/p-STAT3蛋白/熟地黄/山茱萸/山药Key words
Jingui Shenqi pill/renal fibrosis/unilateral ureteral obstruction rats/p-STAT3/prepared radix rehmanniae/semen corni/Chinese yam分类
医药卫生引用本文复制引用
刘春燕,朱伟,唐群,梅文娟,陆苗苗,丁志高..金匮肾气丸对单侧输尿管梗阻大鼠肾组织p-STAT3蛋白的影响[J].湖南中医药大学学报,2016,36(10):22-26,5.基金项目
国家自然科学基金青年项目（81503442）；国家自然科学基金青年项目（81603470）；湖南省教育厅科研项目（11C0953）。 （）
