湖南中医药大学学报2016，Vol.36Issue(10)：31-34,4.DOI:10.3969/j.issn.1674-070X.2016.10.008
愈痫灵方对难治性癫痫大鼠多药耐药相关蛋白MRP1、P-gp、LAP、MCP-1表达的作用
Effects of YuXianLing Decoction on the Expressions of Multiple Drug Resistant Related Proteins MRP1, P-gp, LAP MCP-1 in Refractory Epileptic Rats Induced by KA
摘要
Abstract
〔Abstract〕 Objective To investigate the effects of YuXianLing Decoction (YXLD) on the expressions of multi-drug resistance related protein 1 (MRP1), P-glycoprotein(P-gp), layer ankylosis protein(LAP) and Monocyte chemo attractant protein-1 (MCP-1) in hippocampus and temporal lobe cortex of the refractory epileptic rats induced by kaicic acid (KA). Methods (1) Rats models: Hippocampus located by brain stereotactic apparatus according to Paxions Watras map was micro injected 1.0 μL KA to kindle seizures. The rats with above seizure behavior surpass Ⅳ level were intragastric administration interfered by sodium valproate and carbamazepine for 14 days and rekindled with 0.5 μL KA. The rats with above seizure behavior surpass Ⅳ level and epileptiform discharge waves were selected to be successful resistant refractory epilepsy model rats. (2) Grouping and handling: The model rats were randomLy divided into YXLD group, Lamotrigine (LTG)control group and model group, and the sham-operation group and blank control group were also setted. The rats of all groups were intragastric administration interfered by the same volume gavage (2 mL/d) of distilled water, LTG and YXLD for 30 days, respectively. (3) Disposition and detection of specimen: the expressions of MRP1, P-gp, LAP, MCP-1 were detected by immunohistochemical technique. Results Compared with the blank control group and sham-operation group, the expression of successful model rats of MRP1, P-gp, LAP and MCP-1 in hippocampus and temporal cortex were significantly increased,the difference was statistically significant (P<0.01). Compared with the model group, YXLD and LTG intervention can significantly reduce the expression level of MRP1 and P-gp in hippocampus area (P<0.05). The expression of LAP and MCP-1 in hippocampus and temporal lobe cortex were not statistically significant (P>0.05). There were no significant differeces between YXLD group and LAP group (P>0.05). The expressions of LAP, MCP-1 in temporal cortex among groups showed no significant difference (P>0.05). Conclusion The expression of MRP1, P-gp, LAP, MCP-1 of multiply drug resistance in hippocampus and temporal lobe cortex areas of model rats were significantly increased. Reverse and decrease the expression of MRP1 and P-gp in the hippocampal area may be one of the mechanisms of anti-drug resistant epilepsy of YXLD.关键词
耐药性癫痫/愈痫灵方/多药耐药蛋白1/P糖蛋白/红藻氨酸/海马Key words
drug resistance epilepsy/YuXianLing Decoction/multiply drug resistant related protein 1/P-glycoprotein/kainic acid/hippocampus分类
医药卫生引用本文复制引用
李振光,宋祖丽,王净净,李智雄,谢静涛,左亚杰,张曦,肖瑶..愈痫灵方对难治性癫痫大鼠多药耐药相关蛋白MRP1、P-gp、LAP、MCP-1表达的作用[J].湖南中医药大学学报,2016,36(10):31-34,4.基金项目
湖南省教育厅重点科研课题（11A084）；湖南省科学技术厅科技计划一般项目（2012SK3132）。 （）
