湖南中医药大学学报2016，Vol.36Issue(10)：35-38,4.DOI:10.3969/j.issn.1674-070X.2016.10.009
补阳还五汤提取过程中最佳提取时间和药材粉碎度的研究
The Research of Optimum Extraction Time and Degree of Crushing Herbs of Buyang Huanwu Decoction
摘要
Abstract
Objective To determine the optimum extraction time and particle size of Buyang Huaiwu Decoction (BYHWD). Methods The damage of different grinding degrees BYHWD cell wall was determined by microscopy and the active ingredients dissolution situation herbs broken were analyzd by HPLC. Results The microstructure of herbs at 80 mesh to 100 mesh was messy and irregular. Herbs cell structure was incomplete because of broking of cell wall. In the middle of the extraction process, the common peaks of active ingredients from different grinding degrees herbs are the most. The similarity of different fingerprints was basically above 0.85 at the extraction time of 300 min. Conclusion The dissolution of BYHWD is stable at 20 mesh/240 min, 40 mesh/180min, 60 mesh/120 min, 80 mesh/90 min, 100 mesh/30 min and decoction pieces and the extraction effect is best.关键词
补阳还五汤/提取时间/粉碎度/显微鉴别/HPLCKey words
Buyang Huanwu Decocotion/extraction time/grinding degree/microscopic identification/HPLC分类
医药卫生引用本文复制引用
唐宇,胡超,杨娇,范诗琪,贺福元..补阳还五汤提取过程中最佳提取时间和药材粉碎度的研究[J].湖南中医药大学学报,2016,36(10):35-38,4.基金项目
国家自然科学基金资助项目（81073142，81173558）。 （）
