湖南中医药大学学报2016，Vol.36Issue(10)：76-81,6.DOI:10.3969/j.issn.1674-070X.2016.10.020
穴位埋药线对难治性癫痫大鼠癫痫样波的发放及大脑海马和颞叶皮质多药耐药相关蛋白MRP1、P-gp表达的影响
Effects of Acupoint Embedding Therapy on Epileptiform Wave of Intractable Epilepsy Rats and the MRP1, P-gp Protein in Hippocampus and Temporal Cortex
摘要
Abstract
〔Abstract〕 Objective To discussion the acupoint embedding therapy on epileptiform wave of intractable epilepsy rats and the multidrug resistance protein 1 (MRP1)、 P-gp protein in hippocampus and temporal cortex. Methods (1) Making models:The rats were injected with kainic acid (KA) in hippocampus region, which kinded and rekinded of sub-convulsant dose to making model. The rats with EEG epileptiform wave are screened as successful modeling refractory epilepsy rats. (2) Grouping and treatment: Buried ordinary wire line group (PTX group) and the drug line group (YX group) were firstly buried side points, spaced 15 days buried in the contralateral acupoints. Lamotrigine (LTG) group rats were fed 2 times a day. The model group were administered the same volume gavage of distilled water for 30 days. (3) The changes of EEG basic rhythm amplitude and frequency were detectd by using VEEG-1518K type digital video EEG monitoring and analysis system. (4) Selecting and detecting specimens: The expressions of multi-resistant related protein MRP1 and P-gp in the hippocampus and temporal lobe cortex were detected. Results The expressions of each group before treatment had no significant difference (P>0.05). After treatment, The YX group and LTG group were compared with Model group and PTX group, respectively, the difference was statistically significant (P<0.05), while YX group and LTG group had no significant difference (P>0.05). Compared with model group, LTG group and YX group can reduce epileptiform EEG wave discharge duration and frequency of issuance in epileptic rats and the multidrug resistance protein MRP1, P-gp expression level in hippocampus and temporal cortex (P<0.05 or P<0.01), the difference between the YX group and PTX group was statistically significant (P<0.05). Conclusion (1) Embedding medicine can short the EEG wave discharge duration time and reduce the frequency of epileptic rats induced by KA seizures. (2) The expression of multidrug resistance related prontein MRP 1 and P-gp in hippocampus were obviously higher than that in cortical areas of KA induced epileptic rats. (3) The reversal and reduction of the expression levels of multidrug resistance gene hippocampus of epileptic rats may be one of the mechanisms of embedding medicine on anti-epilepsy.关键词
穴位埋药线/难治性癫痫/癫痫样波/MPP1/P-gpKey words
acupoint embedding/refractory epilepsy/epilepsy wave/MRP1/P-gp分类
医药卫生引用本文复制引用
宋祖丽,李振光,王净净,李智雄,谢静涛,左亚杰,张曦,肖瑶..穴位埋药线对难治性癫痫大鼠癫痫样波的发放及大脑海马和颞叶皮质多药耐药相关蛋白MRP1、P-gp表达的影响[J].湖南中医药大学学报,2016,36(10):76-81,6.基金项目
湖南省教育厅重点科研课题（11A084）；湖南省科学技术厅科技计划一般项目（2012SK3132）。 （）
