海洋科学2016，Vol.40Issue(8)：27-34,8.DOI:10.11759/hykx/20141231001
脊尾白虾C-型凝集素基因cDNA全长的克隆和表达分析
Cloning and expression analysis of C-type lectin gene ofExo-palaemon carinicauda
摘要
Abstract
Based on a hemocyte cDNA library ofExopalaemon carinicauda generated in our laboratory, the C-type lectin cDNA ofE. carinicauda (namedEcCTL) was cloned by rapid amplification of cDNA ends.EcCTL was 1285-bp long, containing an open reading frame of 1041 bp and encoding a mature protein of 346 amino acids, with a molecular mass of 38.56 kDa. Homology analysis revealed that its amino acid sequence was highly conserved, with homologs in other crustaceans. The degree of similarity ofEcCTL with cDNA ofPalaemon modestuswas 91%. EcCTL expression levels in different tissues were analyzed using quantitative real-time PCR. Its expression was detected in all tested tissues, including hemocytes, gill, hepatopancreas, muscle, ovary, intestine, stomach, and eye-stalk, with the highest expression level in the hepatopancreas. After challenge withVibrio anguillarum and white spot syndrome virus,EcCTL expression was upregulated in the hemocytes and hepatopancreas. Our results imply that EcCTL plays an important role in the immune response in prawns and lays a foundation for studies on the E. carinicaudaimmune system.关键词
脊尾白虾(Exopalaemon carinicauda)/C-型凝集素/基因克隆/基因表达Key words
Exopalaemon carinicauda/C-type lectin/gene cloning/gene expression分类
农业科技引用本文复制引用
王有昆,刘萍,李吉涛,李健..脊尾白虾C-型凝集素基因cDNA全长的克隆和表达分析[J].海洋科学,2016,40(8):27-34,8.基金项目
国家虾产业技术体系(CARS-47) （CARS-47）
山东省泰山产业领军人才工程项目(LNJY2015002) （LNJY2015002）
国家自然科学基金(31472275) （31472275）
青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02)@@@@Modern Agro-industry Technology Research System, No. CARS-47 （2015ASKJ02）
The Program of Shandong Leading Tolent, No. LNJY2015002 （）
National Natural Science Foundation of China, No.31472275 （）
Scien-tific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology , No.2015ASKJ02。 （）
