集美大学学报（自然科学版）2016，Vol.21Issue(5)：338-344,7.
重组褐藻胶裂解酶酶解工艺的优化及产物分析
Optimization of the Enzymatic Process and Analysis of the Hydrolysates by the Recombinant Alginate Lyase
摘要
Abstract
The aim of the study is to determine the dynamic process and technical conditions of alginate degradation by the recombinant alginate lyase from Pseudomonas syringae. The contents of the reducing sugar and the total polysaccharide were determined by 3 , 5-dinitrosalicylic acid assay and phenol-sulfuric acid method, respectively. According to the ratio of reducing sugar and total sugar, the variation regularity of av⁃erage polymeric degree of the enzymatic hydrolysates were obtained. The influence factors in the enzymatic process were studied, and the optimal conditions for alginate degradation by the recombinant alginate lyase were determined. The results showed that optimal conditions for enzymatic hydrolysis were: temperature 25 ℃, pH 7�5, initial substrate concentration 7 g/L, enzyme 0�48 U, and reaction time 210 min. Under these condition, the concentration of reducing sugar reached 0�878 g/L and the average polymetric degree fell to 3 . The enzymatic hydrolysates were identified by mass spectrometer and the final products mainly included monosaccharide, disaccharide and tetrasaccharide.关键词
褐藻胶/酶解/工艺优化/褐藻胶寡糖/聚合度Key words
alginate/enzymolysis/process optimization/alginate oligosaccharides/degree of polymer-ization分类
农业科技引用本文复制引用
王新侠,乔超超,倪辉,肖安风,蔡慧农,朱艳冰..重组褐藻胶裂解酶酶解工艺的优化及产物分析[J].集美大学学报（自然科学版）,2016,21(5):338-344,7.基金项目
福建省自然科学基金资助项目 （）
