临床口腔医学杂志2016，Vol.32Issue(10)：582-585,586,5.DOI:10.3969/j.issn.1003-1634.2016.10.002
10％纳米羟基磷灰石聚醚醚酮浸提液培养成骨细胞（MG63）Wnt3a／β－catenin信号通路的表达
Effects of PEEK/HA composite materials on MG63 cells
摘要
Abstract
Objective:To study the variety of Wnt3a /β-catenin signaling pathway’s characteristic proteins after MG63 cell cultured with 10%nano-hydroxyapatite PEEK composites extracting solution,and evaluate the biocompatibility of composites.Methods:Wnt3a constantly have taken effect in MG63 cells cultured by 10% HA /SPEEK /PEEK extracting solution.Immunofluorescence staining technique qualitatively detected the presence of β-catenin protein in wnt signaling pathway.Western-Blotting detected relatively quantity of β-catenin protein.Real-time PCR technology detected downstream <br> genes c-myc, cyclinD1 expression of signal pathway.Results:Immunofluorescence staining and Western-Blotting results showed that Wnt3a taked effect in MG63 cells cultured by 10% HA /SPEEK /PEEK extracting solution.And β-catenin protein stained green, located in the nucleus with a significant increase in expression.Real-time quantitative PCR results showed that Wnt3a taked effect in MG63 cells cultured by 10%HA /SPEEK /PEEK extracting solution.The expression of downstream target genes in c-myc,cyclinD1 of signaling pathway was significantly increased,and Wnt signaling pathway was opened.Conclusion:10%HA /SPEEK /PEEK for MG63 cells have good osteogenesis,and could been safely used in clini-cal studies.关键词
10%HA/SPEEK/PEEK/MG63成骨细胞/Wnt3a/β-catenin/信号通路Key words
PEEK/HA composite/MG63 Osteoblasts/Wnt3a/β-catenin/Signaling pathway分类
医药卫生引用本文复制引用
任英华,刘学,倪卓,王杨,谢安琪..10％纳米羟基磷灰石聚醚醚酮浸提液培养成骨细胞（MG63）Wnt3a／β－catenin信号通路的表达[J].临床口腔医学杂志,2016,32(10):582-585,586,5.基金项目
深圳市科技计划项目（JCYJ20140411094009912）深圳市南山区科技计划项目 （）
