临床眼科杂志2016，Vol.24Issue(5)：455-458,4.DOI:10.3969/j.issn.1006-8422.2016.05.024
葛花总黄酮对糖尿病小鼠视网膜形态学改变及 VEGF表达的影响
Effects of total flavonoids extracted from puerariae flos on retinal morphology and VEGF expression in dia-betic mice
摘要
Abstract
Objective To investigate the effects of total flavonoids extracted from Puerariae Flos on retina morphol-ogy and vascular endothelial growth factor ( VEGF) expression in diabetic retinopathy mouse.Methods Forty C57BL/6J mice were randomly divided into 5 groups:control group ( Control) , diabetic group ( DM) , low dose total flavonoids ( TFF-I) , middle-dose group ( TFF-II) and high-dose group ( TFF-III) .Type 1 diabetic mouse model was generated by intraper-itoneal injected of STZ.TFF treatment began from 5 weeks after STZ injection.When TFF treatment completed, retina tis-sue was collected and stained for morphological studies.Results In low-and middle-dose TTF groups, retina was slightly thin, and the thickening of capillary basement membrane was not obvious.Ganglion cell number slightly reduced.Mild vacuolar degeneration can be observed in nerve fiber layer.Cells in inner and outer nuclear layer cells were largely in or-der.In TFF high-dose group, retinal thickness was normal.Ganglion cell number slightly reduced.Vacuolar degeneration was minimal, and cells were arranged orderly in inner and outer nuclear layers.VEGF weakly expressed in TFF treatment groups.Average optical density of VEGF positive products in low, middle and high-dose groups were 35.53 ±3.12, 25.42 ±3.60, and 16.43 ±4.89, respectively.These values were significantly lower than that in diabetic group (55.54 ±8.87;P <0.01).Conclusions VEGF plays an important role in development of diabetic retinopathy.Total flavonoids from Pu-erariae Flos can improve the progress of diabetic retinopathy in mice by decreasing the expression of VEGF.关键词
葛花总黄酮/糖尿病视网膜病变/视网膜形态/血管内皮生长因子Key words
Total flavonoids from Puerariae Flos/Diabetic retinopathy/Retinal morphology/Vascular endothelial growth factor引用本文复制引用
杨芳,白晨,余锦强,李凌,谭可,李岱..葛花总黄酮对糖尿病小鼠视网膜形态学改变及 VEGF表达的影响[J].临床眼科杂志,2016,24(5):455-458,4.基金项目
湖北省科技厅基金资助项目（2008ABA380）；湖北省教育厅基金资助项目 （）
