生态与农村环境学报2016，Vol.32Issue(5)：774-779,6.DOI:10.11934/j.issn.1673-4831.2016.05.013
有机肥与化肥配施对黄瓜产量及土壤微生物多样性的影响
Effect of Combined Application of Chemical Fertilizer With Organic Manure on Cucumber Yield and Soil Micro-bial Diversity
摘要
Abstract
Combined application of chemical fertilizer with organic manure has obvious advantages over application of chemical fertilizer or organic manure alone in terms of crop yield, but little has been reported about effect of the combina⁃tion on soil microbial diversity. A greenhouse experiment was conducted to investigate effects of the combined application of chemical fertilizer with organic manure on cucumber yield and soil microbial diversity. The experiment was designed to have 5 treatments, that is, Treatment CK (no fertilization), Treatment CF [chemical fertilizer, w(N) ∶ w(P2O5) ∶ w (K2O)=15 ∶ 15 ∶ 15], Treatment WOF (NPK +water hyacinth), Treatment POF (NPK + pig manure) and Treatment FOF ( NPK + fermented bedding) . All the treatments, except for Treatment CK, were the same in N input and the combi⁃nation followed 1 ∶ 1 ratio. Results show that the treatments of combined application were higher than or equal to Treatment CF in cucumber yield. Treatment FOF in particular was 26.77% higher, and followed by Treatment WOF. The combined fertilization was found to be able to maintain or raise cucumber yield and improve quality of the fruit as well. The cucum⁃bers from the treatments of combined fertilization were about 30% higher than those from Treatment CF in Vitamin C con⁃tent. The combined fertilization also improved nitrogen use efficiency, but the effect varied sharply from treatment to treat⁃ment. PCR-DGGE shows that soil bacteria community structure in the five treatments could be classified into three groups. Treatments CK and CF were quite similar in soil bacterial community structure and Treatments WOF and POF were similar too, while Treatment FOF was unique. The treatments of combined fertilization, regardless of source of organic manure, all increased the Shannon⁃Wiener index and abundance of soil bacteria by as far as over 5% as compared with Treatment CF,which indicates that application of extraneous organic matter could alter structure of the soil microbial community and im⁃prove soil microbial diversity, but the application of chemical fertilizer ( CF) has little effect on the soil microbial commu⁃nity structure.关键词
有机肥/化肥/肥料配施/黄瓜/土壤微生物多样性Key words
organic manure/chemical fertilizer/combined fertilization/cucumber/soil microbial diversity分类
资源环境引用本文复制引用
罗佳,刘丽珠,王同,严少华,卢信,范如芹,张振华..有机肥与化肥配施对黄瓜产量及土壤微生物多样性的影响[J].生态与农村环境学报,2016,32(5):774-779,6.基金项目
公益性行业（农业）科研专项（201203050）；江苏省农业科技自主创新资金〔CX（15）1003〕 （）
