农药学学报2016，Vol.18Issue(5)：567-574,8.DOI:10.16801/j.issn.1008-7303.2016.0079
杨树炭疽病菌对多菌灵及3种 DMIs杀菌剂的敏感性
Sensitivities of poplar anthracnose fungi isolates to carbendazim and three C-14α-demethylation inhibitors
摘要
Abstract
Sensitivities of 59Colletotrichum gloeosporioides isolates and fourC. aenigma isolates causing poplar anthracnose to four fungicides (carbendazim, difenoconazole, tebuconazole and triadimefon) were determined by measuring the mycelium growth. The mean and the range of EC50 values of 59C. gloeosporioides isolates were (0.066 4 ± 0.013 1) and 0.037 1-0.130 1 μg/mL for carbendazim, (0.374 1 ± 0.254 8) and 0.102 5-1.680 μg/mL for difenoconazole, (0.681 2 ± 0.442 1) and 0.069 1-1.917 μg/mL for tebuconazole, and (19.82 ± 6.200) and 3.053-38.59 μg/mL for triadimefon. The frequency distribution of EC50 values ofC. gloeosporioides for carbendazim was described by continuous, unimodal curve, indicating the absence of carbendazim-resistant subpopulation among these isolates. The frequency distribution of EC50 values ofC. gloeosporioides for difenoconazole and tebuconazole was bimodal curves, implying the appearance of resistant subpopulation. The highest EC50 values were 1.33-, 13.19-, 13.66-, and 2.14-times greater than the lowest values for carbendazim, difenoconazole, tebuconazole and triadimefon in the case of fourC. aenigma isolates. The EC50 values ofC. aenigma isolate Ca-4 to difenoconazole and tebuconazole were higher than 4 μg/mL. There was no significantly difference among the sensitivities ofC. gloeosporioides isolates from different poplar hosts (P > 0.05). Spearman’s rank correlation analysis showed that there was positive correlation between sensitivities ofC. gloeosporioides isolates for difenoconazole and tebuconazole (ρ = 0.665 5,P <0.000 1), and there was weakly positive correlation between sensitivities ofC. gloeosporioides isolates for tebuconazole and triadimefon (ρ = 0.489 6,P < 0.000 1). However, no correlation between other fungicides was observed. The results will be an important reference for chemical control of poplar anthracnose.关键词
杨树炭疽病/胶孢炭疽菌/炭疽菌/多菌灵/C-14α-脱甲基酶抑制剂 (DMIs)Key words
poplar anthracnose/Colletotrichum gloeosporioides/Colletotrichum aenigma/carbendazim/C-14α-demethylation inhibitors (DMIs)分类
农业科技引用本文复制引用
宋丹丹,张伊莹,张琳婧,王毓,李肖宇,刘西莉,陈磊,田呈明..杨树炭疽病菌对多菌灵及3种 DMIs杀菌剂的敏感性[J].农药学学报,2016,18(5):567-574,8.基金项目
中国博士后科学基金面上项目(2015M581004)；北京市支持中央高校在京高校共建项目--科学研究与研究生培养项目(2050205) （2015M581004）
