农药学学报2016，Vol.18Issue(5)：575-581,7.DOI:10.16801/j.issn.1008-7303.2016.0081
中国华北地区瓜类尖孢镰刀菌对咪鲜胺的敏感性及抗药突变株生物学性状研究
Sensitivity to prochloraz and main biological characteristics of resistant mutants ofFusarium oxysporum from cucurbitaceous crops in North China
摘要
Abstract
The present study was performed to explicit sensitivity ofFusarium oxysporumSchl. on cucurbitaceous crops from North China and clarify the biological characteristics of the resistant mutants. The sensitivities of 112 isolates ofFusarium oxysporumon cucurbitaceous crops to prochloraz were determined by mycelial growth rate method. And the isolates were collected mainly from areas in Beijing, Shandong and Hebei provinces where no azoles fungicides had been used before. Resistant mutants to prochloraz were acquired by fungicide adaptation test. The results showed that the frequency of isolates with different sensitivity to prochloraz distributed as a unimodal curve, and there was no sub-group with declining sensitivity. The mean EC50 value was (0.030 1 ± 0.030 4) μg/mL and it could be considered as the sensitivity baseline. Seven resistant mutants ofF. oxysporum to prochloraz were obtained with the resistance factors ranging from 6.2-26.8 fold. The growth rate of mycelium on PDA between the mutants and the parent isolates, the dry weight of mycelium, and the virulence of 7 resistant mutants were significantly lower than that of the parent isolates. Only the resistance of mutant HG13052701-R1 could be stably inherited, while the other 6 mutants could not be inherited stably. Cross-resistance existed only between prochloraz and tebuconazole, and there is no cross-resistance found among prochloraz, carbendazim and hymexazol. The results suggested that resistant mutant ofF. oxysporum on cucurbitaceous crops can be developed under the selection of prochloraz, and the resistance risk is low.关键词
尖孢镰刀菌/咪鲜胺/敏感性/抗药性突变株/生物学性状Key words
Fusarium oxysporum/prochloraz/sensitivity/resistant mutants/biological characteristics分类
农业科技引用本文复制引用
裴龙飞,朱发娣,柴阿丽,石延霞,谢学文,李宝聚..中国华北地区瓜类尖孢镰刀菌对咪鲜胺的敏感性及抗药突变株生物学性状研究[J].农药学学报,2016,18(5):575-581,7.基金项目
国家科技支撑计划(2012BAD19B06)；国家大宗蔬菜产业技术体系(CARS-25)；农业部园艺作物遗传改良重点开放实验室；中国农业科学院科技创新工程(CAAS-ASTIP-CAAS) （2012BAD19B06）
