农药学学报2016，Vol.18Issue(5)：589-595,7.DOI:10.16801/j.issn.1008-7303.2016.0083
低剂量乙基多杀菌素对小菜蛾解毒酶的影响
Effect of low dose spinetoram on detoxification enzymes inPlutella xylostella
摘要
Abstract
To clarify the effects of low dose spinetoram on the activitiy of detoxifying enzymes in Plutella xylostella (L.), the toxicities of spinetoram and spinosad to susceptible population ofP. xylostella were assayed using leaf dipping method. And the changing dynamics of detoxifying enzymes of carboxylesterase, glutathioneS-transferase and mixed-functional oxidase were measured after exposure to LC25 and LC50 doses of spinetoram at 6, 12, 24, 48 and 72 h, respectively. The results showed that the spinetoram had higher toxicity toP. xylostella than that of spinosad. The 48 h LC25 and LC50 of spinetoram were 0.018 and 0.048 mg/L, respectively. After treatment with low concentrations spinetoram, a large fluctuation of the CarE was observed. CarE activity in the treatment strain were higher than those in the untreated control strain after treatment 6-24 h, and showed tendency of first increasing and then decreasing. Whereas, from 24 h to 72 h, CarE activity was lower than that, and with a certain amount of time effect onP. xylostella. GST had obviously induced effect. And GST activity was higher than that of the control group. MFOs activities had been distinctly inhibited which were significantly lower than that of the control group, whereas they were similar at 48 h. These results indicated that GST may be the major detoxifying enzyme for metabolizing spinetoram inP. xylostella.关键词
低剂量/乙基多杀菌素/小菜蛾/解毒酶Key words
low dose/spinetoram/Plutella xylostella/detoxification enzymes分类
农业科技引用本文复制引用
田雪莲,尹显慧,龙友华,李明,蔡滔,李荣玉,朱流红..低剂量乙基多杀菌素对小菜蛾解毒酶的影响[J].农药学学报,2016,18(5):589-595,7.基金项目
国家自然科学基金项目(31460481)；贵州大学引进人才科研项目[贵大人基合(2008)030]；贵州大学研究生创新基金(研农2016029) （31460481）
