山东医药2016，Vol.56Issue(40)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.40.001
RNAi沉默 Rac1基因对结肠癌细胞增殖及裸鼠成瘤的影响
Effects of RNAi-mediated knockdown of Rac1 gene on growth and transplanted tumors of colon cancer SW480 cells in nude mice
摘要
Abstract
Objective To investigate the effects of Rac1 gene silenced by RNA interference (RNAi) on cell prolifera-tion and growth of subcutaneous tumor of colon cancer SW480 cells in nude mice.Methods ① Cell experiment: colon cancer SW480 cells cultured in vitro were randomly divided into the blank control group, negative control group, and shRNA-Rac1 group.Recombinant lentivirus virus vector ( NC-shRNA and Rac1-shRNA) were constructed and were respectively transfected into negative control group and shRNA-Rac1 group.The expression levels of Rac1 mRNA and protein were meas-ured by quantitative real-time polymerase chain reaction ( qRT-PCR) , and Western blotting, respectively.Cell proliferation at 24, 48, 72, and 96 h after transfection was determined by MTT.②Animal experiment:cells from shRNA-Rac1 group and negative control group were planted into nude mice to establish mice models of colon cancer ( observation group and control group) .Formulation and growth of the tumor was observed.After the mice were sacrificed by cervical dislocation, tumor tis-sues were removed completely and we measured its weight and size.Meanwhile, Rac1expression was also detected by immu-nohistocytochemistry.Results ①cell experiment:Compared with the blank control group and negative control group, the expression of Rac1 mRNA and protein in the shRNA-Rac1 group decreased significantly (all P<0.05).The proliferation a-bility of colon cancer cells was significantly inhibited in the shRNA-Rac1 group at all detecting time points as compared with that of the control group (all P<0.05).②Animal experiment:tumor was observed in all cases of the control group 4-8 days after the transplantation.While in the observation group, tumor was only observed in 2 cases 35 days after the transplanta-tion, and it took 10 days longer to form a tumor than that in the control group.On the 35th day of transplantation, average volume of the tumor in the observation group and control group was (948.13 ±523.50) and (32.54 ±43.13) mm3 , and the tumor average weight was respectively (0.873 ±0.372) and (0.023 ±0.031) g, and significant difference was found be-tween the two groups (all P<0.01).Besides, compared with the control group, the expression rate of Rac1 in the observa-tion group was significantly lower than that in the control group (P<0.01).Conclusion Silencing the expression of Rac1 decreases the proliferation of colon cancer cells SW480 and inhibits the tumor formation in nude mice.关键词
结肠癌/Rac1/RNA干扰/裸鼠成瘤/细胞增殖Key words
colon cancer/Rac1/RNA interference/tumor growth in nude mice/cell proliferation分类
医药卫生引用本文复制引用
解娜,黄幼生,罗志飞,薛逢贵..RNAi沉默 Rac1基因对结肠癌细胞增殖及裸鼠成瘤的影响[J].山东医药,2016,56(40):1-4,4.基金项目
国家自然科学基金资助项目（81260321）。 （）
