山东医药2016，Vol.56Issue(40)：17-20,4.DOI:10.3969/j.issn.1002-266X.2016.40.005
利拉鲁肽对非酒精性脂肪肝大鼠IR 及 SOCS-3表达的影响
Effects of liraglutide on insulin resistance and SOCS-3 expression in rats with nonalcoholic fatty liver disease
摘要
Abstract
Objective To investigate the effects of liraglutide ( Lira) on insulin resistance and expression of SOCS-3 in rats with nonalcoholic fatty liver disease ( NAFLA) .Methods Twenty NAFLD rat models were prepared and then ran-domly divided into high fat group ( HF) and HF+Lira group.Meanwhile, another 10 normal rats were selected as the nor-mal control ( NC) group.HF group, HF+Lira group were given high fat diet for 16 weeks.After 12 weeks of high fat feed-ing in HF+Lira group, 600μg/( kg· d) Lira was administered by intraperitoneal injection for 4 weeks.At the end of the 16 weeks, the rats were killed.The changes of liver pathology were observed by optical microscope.The serum levels of al-anine aminotransferase (ALT), aspartate aminotransferase (AST), total triglyceride (TG), cholesterol (TC) and fasting blood glucose ( FPG) were detected by automatic biochemical analyzer.The fasting insulin ( FINS) was determined by ra-dioimmunoassay, and insulin resistance index ( HOMA-IR) was assessed by homeostasis mode assessment.The liver ex-pression of SOCS-3 mRNA was detected by RT-PCR.The relationship between SOCS-3 mRNA and HOMA-IR was ana-lyzed.Results Compared with NC group, liver index, HOMA-IR, and the levels of ALT, AST, TG, TC, FPG and FINS were significantly increased in HF group ( all P<0.05); compared with HF group, the above indexes of the HF+Lira group were all decreased, and the difference was statistically significant (all P<0.05).No abnormal pathological changes in the liver tissues of the NC group.In the HF group, the liver cells were arranged in disorder, there were more lipid drop-lets in the cells, and some hepatocytes degenerated and had inflammatory cells infiltration.The hepatic steatosis in the HF +Lira group was lighter than that in the HF group.The expression level of SOCS-3 mRNA in HF group was significantly higher than that in NC group and the HF+Lira group (all P<0.05);and no significant difference was found between the NC group and the HF+Lira group (P>0.05).HOMA-IR was positively correlated with of the expression of SOCS-3 mR-NA (r=0.445, P<0.05).Conclusion Lira has certain effect in treatment of NAFLD, whose mechanism may be relat-ed to the decrease of IR and inhibition of SOCS-3 mRNA expression.关键词
非酒精性脂肪肝/胰岛素抵抗/胰高血糖素样肽1/细胞因子信号转导抑制物3Key words
nonalcoholic fatty liver/insulin resistance/glucagon-like peptide 1/suppressor of cytokine signaling 3分类
医药卫生引用本文复制引用
侯洪涛,裘艳梅,张建,胡义亭,王玉珍..利拉鲁肽对非酒精性脂肪肝大鼠IR 及 SOCS-3表达的影响[J].山东医药,2016,56(40):17-20,4.基金项目
河北省医学科学研究重点课题计划（ZL20150117）。 （）
